Walsh Ryan, DeRosa Maria C
Chemistry Department, Carleton University, 1125 Colonel By Drive, Ottawa, Ont., Canada K1S 5B6.
Biochem Biophys Res Commun. 2009 Oct 30;388(4):732-5. doi: 10.1016/j.bbrc.2009.08.084. Epub 2009 Aug 20.
While it is generally accepted that the functional tertiary structures formed by RNA cannot be replicated by a deoxy version of the same sequence, here we demonstrate conservation of function for a DNA homolog of an RNA aptamer. Using fluorescence anisotropy experiments, this work demonstrates that the all-DNA version of the RNA dopamine aptamer is able to bind dopamine with improved affinity and similar specificity relative to the RNA aptamer. Mutation studies suggest that the binding site is maintained in both structure types. These findings will help to elucidate what sequences and secondary structures allow for retention of function in both RNA and DNA.
虽然人们普遍认为RNA形成的功能性三级结构不能被相同序列的脱氧版本复制，但在此我们证明了RNA适体的DNA同源物的功能保守性。通过荧光各向异性实验，这项工作表明RNA多巴胺适体的全DNA版本能够以比RNA适体更高的亲和力和相似的特异性结合多巴胺。突变研究表明两种结构类型中结合位点均得以保留。这些发现将有助于阐明哪些序列和二级结构能够使RNA和DNA都保留功能。
