Magnusson U
Department of Obstetrics and Gynaecology, College of Veterinary Medicine, Uppsala, Sweden.
Vet Immunol Immunopathol. 1991 Apr;28(2):117-26. doi: 10.1016/0165-2427(91)90134-x.
The in vitro effect of oestradiol-17 beta (Oe2) on cell-mediated immunity (CMI) and phagocytosis by leukocytes obtained from ten and eight sows, respectively, was studied. The concentrations of Oe2 used were 300 pmol/l and 3000 pmol/l, to reflect a low and a high prepartum concentration of the hormone. The CMI was measured by lymphocyte proliferation (LP) tests performed on whole blood and purified mononuclear cells (MNC) and by estimation of the production of interleukin-2 (IL-2) in the latter type of culture. In the whole blood LP test, the mitotic response to concanavalin A (Con A) was increased by 29% (P less than or equal to 0.05) when the Oe2 concentration was 300 pmol/l. The higher concentration of Oe2 (3000 pmol/l) increased the amount of IL-2 in supernatants from Con A-stimulated MNC by 17% (P less than or equal to 0.05). The phagocytic function of polymorphonuclear leukocytes was determined in a chemiluminescence assay. In this assay, the maximal rate of phagocytosis was reduced by 12% (P less than or equal to 0.01) and the area under the curve by 10% (P less than or equal to 0.05) at an Oe2 concentration of 3000 pmol/l. The results show that prepartum concentrations of Oe2 can promote CMI in vitro, but weaken phagocytosis by porcine leukocytes.
研究了17β-雌二醇（Oe2）对分别取自10头和8头母猪的白细胞的细胞介导免疫（CMI）和吞噬作用的体外影响。所用的Oe2浓度分别为300 pmol/l和3000 pmol/l，以反映该激素产前的低浓度和高浓度。通过对全血和纯化的单核细胞（MNC）进行淋巴细胞增殖（LP）试验以及估计后一种培养物中白细胞介素-2（IL-2）的产生来测量CMI。在全血LP试验中，当Oe2浓度为300 pmol/l时，对刀豆球蛋白A（Con A）的有丝分裂反应增加了29%（P≤0.05）。较高浓度的Oe2（3000 pmol/l）使Con A刺激的MNC上清液中的IL-2量增加了17%（P≤0.05）。通过化学发光测定法测定多形核白细胞的吞噬功能。在该测定中，当Oe2浓度为3000 pmol/l时，最大吞噬率降低了12%（P≤0.01），曲线下面积降低了10%（P≤0.05）。结果表明，产前Oe2浓度可在体外促进CMI，但会削弱猪白细胞的吞噬作用。
