Magnusson U, Villacres-Eriksson M
Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
Zentralbl Veterinarmed B. 1995 Jul;42(5):277-83. doi: 10.1111/j.1439-0450.1995.tb00711.x.
A bioassay for porcine interleukin-1 (IL-1) was validated. By use of this bioassay, the influence of increasing concentrations of oestradiol-17 beta on IL-1 secretion by endotoxin-stimulated porcine mononuclear cells (MNC) in vitro was studied. Supernatants from cultures of porcine MNC stimulated with LPS (0.1-10 micrograms/ml) induced a proliferative response in the IL-1 and IL-2 dependent cell line D10.G4.1 (D-10) in a dose-dependent manner with respect to LPS. Supernatants of LPS stimulated porcine MNC did not show any IL-2 activity in an bioassay for IL-2. Porcine MNC were cultured in the presence of increasing concentrations of oestradiol-17 beta (42-1500 pmol/l) and stimulated with LPS (10 micrograms/ml) or in the absence of oestradiol and LPS. The IL-1 secretion by the MNC, as determined by the D-10 bioassay, was significantly (P < 0.01) suppressed by the highest oestradiol concentration tested (1500 pmol/l). The results indicate that high physiological concentrations of oestradiol-17 beta may affect the secretion of IL-1 by porcine MNC.
一种用于猪白细胞介素-1（IL-1）的生物测定法得到了验证。利用这种生物测定法，研究了雌二醇-17β浓度增加对体外内毒素刺激的猪单核细胞（MNC）分泌IL-1的影响。用脂多糖（LPS，0.1 - 10微克/毫升）刺激猪MNC培养物的上清液，相对于LPS以剂量依赖方式诱导IL-1和IL-2依赖细胞系D10.G4.1（D - 10）产生增殖反应。在IL-2生物测定中，LPS刺激的猪MNC上清液未显示任何IL-2活性。将猪MNC在浓度不断增加的雌二醇-17β（42 - 1500皮摩尔/升）存在下培养，并用LPS（10微克/毫升）刺激，或在无雌二醇和LPS的情况下培养。通过D - 10生物测定法测定，MNC分泌的IL-1在测试的最高雌二醇浓度（1500皮摩尔/升）下受到显著抑制（P < 0.01）。结果表明，生理浓度较高的雌二醇-17β可能会影响猪MNC分泌IL-1。
