Doronina N V, Ezhov V A, Trotsenko Iu A
Prikl Biokhim Mikrobiol. 2008 Mar-Apr;44(2):202-6.
Optimal conditions for batch cultivation of the obligate methanotroph Methylosinus trichosporium OB3b on methane without superatmospheric pressure were chosen. The yield of absolutely dry biomass after 120 h of growth reached 20 g/l. This biomass contained 30% poly-beta-hydroxybutyrate (PHB) with molecular weight 300 kDa. The growth process included the stages of biomass growth and PHB biosynthesis. The latter stage occurred under nitrogen-deficiency conditions. It was accompanied by an increase in the activity of PHB biosynthesis enzymes (beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase) and the main NAD(P)H producer, methylenetetrahydromethanopterin dehydrogenase. The activity of PHB depolymerase increased insignificantly.
选择了在无超大气压条件下以甲烷为底物分批培养专性甲烷氧化菌 trichosporium OB3b 的最佳条件。生长 120 小时后绝对干生物量产量达到 20 g/l。该生物量含有 30% 的聚-β-羟基丁酸酯（PHB），分子量为 300 kDa。生长过程包括生物量生长和 PHB 生物合成阶段。后一阶段发生在缺氮条件下。伴随着 PHB 生物合成酶（β-酮硫解酶、乙酰乙酰辅酶 A 还原酶和 PHB 合酶）以及主要的 NAD(P)H 产生者亚甲基四氢甲蝶呤脱氢酶的活性增加。PHB 解聚酶的活性增加不显著。
