Chen Y, Ben K, Cao X, Ma L, Shang E, Feng S
Kunming Institute of Zoology, Academia Sinica, China.
Shi Yan Sheng Wu Xue Bao. 1991 Jun;24(2):137-42.
The inbred Balb/c and C57 mice, and the outbred Swiss Webster mice were intragastrointestinally immunized with human sperm antigens. The lymphocytes from the spleen, mesenteric lymph node (MLN), Peyer's patch (PP) and uterus or epididymis were isolated and cultured. The lymphocyte-secreting antisperm IgG and IgA and the antisperm antibodies in the gut wash and serum were determined with enzyme-linked immunosorbent assay (ELISA). In the Balb/c and Swiss Webster mice, the immune responses to sperm have shown to be stronger than that in C57, stronger in female than in male. The antigenicity of sperm membrane extracts seems to be higher than that of whole sperm. Antisperm antibodies secreted by lymphocytes from the epididymis and uterus have demonstrated to be detectable. For stimulation of the local immune response, the intra-PP and intralumina immunizations are more effective than others.
将近交系Balb/c和C57小鼠以及远交系瑞士韦伯斯特小鼠经胃肠道用人精子抗原进行免疫。分离并培养来自脾脏、肠系膜淋巴结（MLN）、派伊尔结（PP）以及子宫或附睾的淋巴细胞。用酶联免疫吸附测定（ELISA）法测定淋巴细胞分泌的抗精子IgG和IgA以及肠道灌洗液和血清中的抗精子抗体。在Balb/c和瑞士韦伯斯特小鼠中，对精子的免疫反应显示比C57小鼠更强，雌性比雄性更强。精子膜提取物的抗原性似乎高于完整精子。已证明可检测到附睾和子宫淋巴细胞分泌的抗精子抗体。对于刺激局部免疫反应，经PP内和腔内免疫比其他方法更有效。
