Estrada A, Laarveld B, Li B, Redmond M J
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada.
Immunol Invest. 1995 Aug;24(5):819-28. doi: 10.3109/08820139509060709.
Synthetic, biotinylated somatostatin-14 (Somatotropin Release-Inhibiting Factor; SRIF) was conjugated to avidin, and the resulting complex incorporated into immune-stimulating complexes (ISCOMS). The ISCOMS were used to study the systemic and mucosal immune responses induced by parenteral and gastrointestinal vaccination. Mice were immunized by intraperitoneal (IP) and intragastric (IG) routes and subsequently by either IP or IG secondary immunizations (groups-IP/IP; IP/IG; IG/IG). Antigen specific IgG and IgA antibody secreting cells (ASC) from the spleen, mesenteric lymph nodes (MLN) and Peyer's patches (PP's) were studied by an enzyme-linked immunospot assay (ELISPOT). Specific proliferative responses of spleen cells to avidin and to SRIF were measured. Immunization IP/IP evoked the highest serum IgG levels to avidin and to SRIF as well as the highest numbers of splenic IgG isotype ASC. The greatest IgA response in MLN and PP's was induced by IP/IG immunization. Only marginal mucosal immunity and no splenic cell specific proliferative responses were found by IG/IG immunization. These results indicate that ISCOMS are an effective delivery system for protein-peptide antigens. The ISCOMS system described elicited systemic and mucosal antibody immune responses, and primed specific proliferative response when administered IP/IG. This offers another approach for the design and delivery of mucosally administered peptide vaccines.
合成的生物素化生长抑素 - 14（促生长激素释放抑制因子；SRIF）与抗生物素蛋白偶联，所得复合物被整合到免疫刺激复合物（ISCOMS）中。ISCOMS用于研究经肠胃外和胃肠道接种诱导的全身和黏膜免疫反应。小鼠通过腹腔内（IP）和胃内（IG）途径免疫，随后通过IP或IG进行二次免疫（组 - IP/IP；IP/IG；IG/IG）。通过酶联免疫斑点测定（ELISPOT）研究来自脾脏、肠系膜淋巴结（MLN）和派伊尔结（PP）的抗原特异性IgG和IgA抗体分泌细胞（ASC）。测量脾脏细胞对抗生物素蛋白和SRIF的特异性增殖反应。IP/IP免疫诱导出最高的针对抗生物素蛋白和SRIF的血清IgG水平以及最高数量的脾脏IgG同种型ASC。IP/IG免疫诱导MLN和PP中最大的IgA反应。IG/IG免疫仅发现边缘性黏膜免疫且未发现脾脏细胞特异性增殖反应。这些结果表明ISCOMS是一种有效的蛋白质 - 肽抗原递送系统。所描述的ISCOMS系统在经IP/IG给药时引发全身和黏膜抗体免疫反应，并引发特异性增殖反应。这为黏膜给药的肽疫苗的设计和递送提供了另一种方法。
