Baggesen D L, Olsen J E, Bisgaard M
National Veterinary Laboratory, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Avian Pathol. 1992 Dec;21(4):569-79. doi: 10.1080/03079459208418878.
Three-hundred-and-eighty-seven strains of Salmonella typhimurium obtained from successive generations of parent stock originating from three different rearing farms were characterized by phage typing and plasmid profiling. Seventy-six strains representing dominant types were selected for restriction enzyme analysis and colony hybridization. The main phage type on each of the three farms was 110. Plasmid profiling, however, allowed further subtyping. All but three isolates carried the serotype-specific virulence-associated plasmid. Restriction enzyme analysis showed variations in this plasmid as well as the presence of co-migrating plasmids of the same size. At each locality one or more clonal lines of S. typhimurium were reisolated from successive generations, indicating that the infections were persistent. Although house construction, sanitation and disinfection procedures, in addition to biosecurity in general, were improved significantly during the observation period, infection with 5. typhimurium was not eliminated until eradication of the beetle Alphitobius diaperinus was complete.
从三个不同饲养场的亲代种群连续几代中获得的387株鼠伤寒沙门氏菌，通过噬菌体分型和质粒图谱分析进行了特征鉴定。选择了代表优势类型的76株菌株进行限制性内切酶分析和菌落杂交。三个饲养场中每个饲养场的主要噬菌体类型均为110。然而，质粒图谱分析允许进一步进行亚型分析。除三株分离株外，所有分离株均携带血清型特异性毒力相关质粒。限制性内切酶分析显示该质粒存在变异，以及存在大小相同的共迁移质粒。在每个地点，从连续几代中重新分离出一株或多株鼠伤寒沙门氏菌克隆系，表明感染持续存在。尽管在观察期内，房屋建设、卫生和消毒程序以及总体生物安全措施都有显著改善，但直到彻底根除甲虫黑菌虫，鼠伤寒沙门氏菌感染才被消除。
