Comparison of the enzyme linked immunosorbent assay, the haemagglutination inhibition test and the agar gel precipitation test for the detection of antibodies against infectious bronchitis and Newcastle disease in commercial broilers.

Serological results as obtained by the agar gel precipitation (AGP) test, haemagglutination inhibition (HI) test (M41, D274, D1466) and an enzyme linked immunosorbent assay (ELISA) from commercial broiler flocks, which had suffered from a clinical infectious bronchitis (IB) infection in the fattening period, were compared with serological findings of control broiler flocks. In addition the Hi-test and ELISA for Newcastle disease (ND) were compared. The AGP results differ significantly between groups. The test had a low sensitivity. The results of the HI-IBV tests were similar for both groups and no difference was seen between flocks with or without clinical IB problems. The IBV ELISA showed a highly significant difference between the two groups. In contrast to the low and not significant correlation coefficients (r) between the IBV HI tests and the ELISA, the value of r between the NDV HI and ELISA was high. The results indicate an ELISA as the preferred test for the diagnosis of IB in broilers.