Ewing M L, Kleven S H, Brown M B
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
Avian Dis. 1996 Jan-Mar;40(1):13-22.
Hemagglutination-inhibition (HI) assay and a new affinity-purified enzyme-linked immunosorbent assay (ELISA) for detection of antibody to Mycoplasma gallisepticum (MG) compared for use as confirmatory tests for the National Poultry Improvement Plan program. Samples from three different poultry populations with different prevalences of MG infection were studied: commercial broiler breeder birds (low prevalence of infection), fair and exhibition birds (moderate prevalence of infection), and experimentally infected birds (high prevalence of infection). Western immunoblots were used to confirm infection status in samples that had discrepancies between HI and ELISA results. The prevalence of infection in commercial broiler birds and in exhibition and fair birds in Florida was determined. Samples from culture-positive Mycoplasma synoviae (MS) flocks also were tested and compared for potential cross-reactions. The prevalence of MG infection was very low (< 1%) in the commercial population, with no significant difference between the HI and ELISA test results (P = 0.3157). The prevalence of MG infection in the fair and exhibition birds tested was approximately 40%, and ELISA was more accurate than HI for confirmation of MG infection in this population (P = 0.036). In birds experimentally infected with MG, there was no significant difference between HI and ELISA results (P = 0.6542). Of the 195 sera collected from flocks confirmed positive for MS by culture, 15% cross-reacted with the MG serum plate agglutination (SPA) test. There were no cross-reactions observed with either the MG ELISA or HI. There was a positive correlation of HI titers to ELISA values (R = 0.621, P < 0.01). Results from this study showed there were no differences between ELISA and HI as confirmatory tests in populations with a low prevalence of MG infection. However, ELISA was superior to HI in a population with moderate levels of MG infection.
血凝抑制（HI）试验和一种新的亲和纯化酶联免疫吸附测定（ELISA）用于检测鸡毒支原体（MG）抗体，对其作为国家家禽改良计划项目的确认试验进行了比较。研究了来自三个不同家禽群体的样本，这些群体中MG感染的患病率不同：商业肉种鸡（感染率低）、参加展会的家禽（感染率中等）和实验感染的家禽（感染率高）。对于HI和ELISA结果存在差异的样本，使用western免疫印迹法确认感染状态。确定了佛罗里达州商业肉鸡以及参加展会的家禽中MG感染的患病率。还对来自滑膜支原体（MS）培养阳性鸡群的样本进行了检测，并比较了潜在的交叉反应。商业群体中MG感染的患病率非常低（<1%），HI和ELISA检测结果之间无显著差异（P = 0.3157）。检测的参加展会的家禽中MG感染的患病率约为40%，在该群体中，ELISA在确认MG感染方面比HI更准确（P = 0.036）。在实验感染MG的家禽中，HI和ELISA结果之间无显著差异（P = 0.6542）。从通过培养确认MS阳性的鸡群中收集的195份血清中，有15%与MG血清平板凝集（SPA）试验发生交叉反应。MG ELISA或HI均未观察到交叉反应。HI滴度与ELISA值呈正相关（R = 0.621，P < 0.01）。本研究结果表明，在MG感染率低的群体中，ELISA和HI作为确认试验没有差异。然而，在MG感染水平中等的群体中，ELISA优于HI。
