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在血清学检测（酶联免疫吸附测定和血凝抑制试验）中使用蛋黄来检测新城疫、传染性支气管炎和鸡毒支原体的抗体。

Use of egg yolk in serological tests (ELISA and HI) to detect antibody to Newcastle disease, infectious bronchitis, and Mycoplasma gallisepticum.

作者信息

Piela T H, Gulka C M, Yates V J, Chang P W

出版信息

Avian Dis. 1984 Oct-Dec;28(4):877-83.

PMID:6098252
Abstract

Serum and yolks from commercial flocks and from hens exposed to Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and Mycoplasma gallisepticum (MG) were tested for immunoglobulin G antibody by the enzyme-linked immunosorbent assay (ELISA) and the hemagglutination-inhibition (HI) test. Yolks prepared by chloroform extraction and low-speed centrifugation performed well in the serological tests used and were a suitable alternative to serum for antibody determination by the ELISA for NDV, IBV, and MG and by HI test for NDV.

摘要

对来自商业鸡群以及感染新城疫病毒（NDV）、传染性支气管炎病毒（IBV）和鸡毒支原体（MG）的母鸡的血清和蛋黄，采用酶联免疫吸附测定（ELISA）和血凝抑制（HI）试验检测免疫球蛋白G抗体。通过氯仿萃取和低速离心制备的蛋黄在所用血清学试验中表现良好，是ELISA法检测NDV、IBV和MG抗体以及HI试验检测NDV抗体时血清的合适替代物。

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Avian Dis. 1984 Oct-Dec;28(4):877-83.
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