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通过免疫过氧化物酶染色法检测组织中的鸭瘟病毒抗原。

Detection of duck plague virus antigen in tissues by immunoperoxidase staining.

作者信息

Islam M R, Nessa J, Halder K M

机构信息

Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

出版信息

Avian Pathol. 1993 Jun;22(2):389-93. doi: 10.1080/03079459308418929.

Abstract

An avidin-biotin-peroxidase method of immunoperoxidase staining was successfully adapted for detection of duck plague virus antigen in formalin-fixed, paraffin-embedded tissue sections of the liver and spleen of experimentally infected domestic ducks. Positive staining was localized mostly in the nucleus but was also present in the cytoplasm of a few hepatocytes and von Kupffer cells of the liver, and lymphocytes and reticular cells of the spleen.

摘要

一种抗生物素蛋白-生物素-过氧化物酶免疫过氧化物酶染色方法成功应用于检测实验感染家鸭肝脏和脾脏的福尔马林固定、石蜡包埋组织切片中的鸭瘟病毒抗原。阳性染色主要定位于细胞核,但也存在于肝脏少数肝细胞和库普弗细胞的细胞质以及脾脏的淋巴细胞和网状细胞中。

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