Franchini Antonella, Casarini Livio, Ottaviani Enzo
Department of Animal Biology, University of Modena and Reggio Emilia, via Campi 213/D, 41100 Modena, Italy.
Chemosphere. 2008 Sep;73(3):267-71. doi: 10.1016/j.chemosphere.2008.06.043. Epub 2008 Jul 30.
The FETAX (frog embryo teratogenesis assay Xenopus) is considered a useful bioassay to detect health hazard substances. In the study of the marine toxin palytoxin (PTX), FETAX has revealed evident impacts on embryo mortality, teratogenesis and growth at the two highest (370 and 37nM) concentrations used. Significant mortality rates, peaks in the number of malformed embryos and delays in growth were found, while the total sample number fell by about 80% at the end of the assay with the concentrated dose. The histological analysis to evaluate the morpho-functional induced modifications demonstrated damage to the nervous and muscle tissue, a general reduction in the size of the main inner visceral organs and severe injury to the heart structure in some specimens. No inflammatory response was observed.
爪蟾胚胎致畸试验（FETAX）被认为是一种用于检测健康危害物质的有用生物测定法。在对海洋毒素岩沙海葵毒素（PTX）的研究中，FETAX显示在所使用的两个最高浓度（370和37纳摩尔）下，对胚胎死亡率、致畸作用和生长有明显影响。发现了显著的死亡率、畸形胚胎数量峰值和生长延迟，而在高剂量试验结束时，总样本数量减少了约80%。用于评估形态功能诱导性改变的组织学分析表明，神经和肌肉组织受损，主要内部内脏器官的大小普遍减小，一些标本的心脏结构严重受损。未观察到炎症反应。
