Ye Xuesong, Wang Peng, Liu Jun, Zhang Shaomin, Jiang Jun, Wang Qingbo, Chen Weidong, Zheng Xiaoxiang
Qiushi Academy for Advanced Studies, Zhejiang University, Hangzhou 310027, PR China.
J Neurosci Methods. 2008 Sep 30;174(2):186-93. doi: 10.1016/j.jneumeth.2008.07.002. Epub 2008 Jul 15.
A portable multi-channel telemetry system which can be used for brain stimulation and neuronal activity recording in freely behaving small animals is described here. This system consists of three major components of headstage, backpack and portable Personal Digital Assistant (PDA). The headstage contains high precision instrument amplifiers with high input impedance. The backpack is comprised of two parts: (1) a main board (size: 36 mm x 22 mm x 3.5 mm and weight: 40 g with batteries, 20 g without), with current/voltage stimulator and special circuit suitable for neuronal activity recording and (2) and a bluetooth transceiver, with a high data transmission rate up to 70 kb/s, suitable for downloading stimulation commands and uploading acquired data. We recorded neuronal activities of the primary motor area of a freely behaving rat with 12-bit resolution at 12 k samples/s. The recorded data and analysis results showed that the system was successful by comparing with the commercial equipment Cerebus 128-Channel Data Acquisition System (Cyberkinetics Inc.). Using the PDA, we can control stimulation and recording. It provides a flexible method to do some research work in the circumstances where other approaches would be difficult or impossible.
本文介绍了一种便携式多通道遥测系统，可用于自由活动的小动物的脑刺激和神经元活动记录。该系统由头端、背包和便携式个人数字助理（PDA）三个主要部件组成。头端包含具有高输入阻抗的高精度仪器放大器。背包由两部分组成：（1）一个主板（尺寸：36毫米×22毫米×3.5毫米，带电池重量：40克，不带电池重量：20克），带有电流/电压刺激器和适合神经元活动记录的特殊电路；（2）一个蓝牙收发器，具有高达70 kb/s的高数据传输速率，适合下载刺激命令和上传采集的数据。我们以12 k采样/秒的12位分辨率记录了自由活动大鼠初级运动区的神经元活动。通过与商业设备Cerebus 128通道数据采集系统（Cyberkinetics公司）比较，记录的数据和分析结果表明该系统是成功的。使用PDA，我们可以控制刺激和记录。它提供了一种灵活的方法，可在其他方法困难或不可能的情况下开展一些研究工作。
