Janssen P H
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
Antonie Van Leeuwenhoek. 1991 Apr;59(3):191-8. doi: 10.1007/BF00580659.
Enrichments on L-tartrate from a freshwater lake sediment yielded a pure culture of anaerobic bacterium designated strain 16Lt1. The rod-shaped organism was motile, did not form spores, and had a gram-negative wall structure. No cytochromes were detected. The mol % G + C of the DNA was 58. The new strain was microaerotolerant, and grew optimally at 30 degrees C and neutral pH in freshwater medium. A wide range of carbohydrates was fermented, with formate, acetate, ethanol, lactate and succinate being the end-products detected. L-tartrate and citrate were fermented to formate, acetate and CO2. L-tartrate was fermented by the dehydratase pathway, and glucose by the Embden-Meyerhof-Parnas pathway. Fumarate was reduced, but nitrate, sulfate, sulfur and thiosulfate were not used as terminal electron acceptors. Glucose metabolism was constitutive, whereas L-tartrate-degrading activity was inducible. When glucose and L-tartrate were both present as substrates, growth was diauxic with glucose being metabolized first. The growth rate and growth yield were higher on glucose than on L-tartrate. Strain 16Lt1 has been deposited with the Deutsche Sammlung von Mikroorganismen as 'Bacteroides' sp. DSM6268.
从一个淡水湖沉积物中对L-酒石酸盐进行富集培养，得到了一种厌氧细菌的纯培养物，命名为16Lt1菌株。该杆状微生物具有运动性，不形成孢子，具有革兰氏阴性细胞壁结构。未检测到细胞色素。DNA的G + C摩尔百分比为58。新菌株耐微氧，在淡水培养基中30℃和中性pH条件下生长最佳。能发酵多种碳水化合物，检测到的终产物有甲酸盐、乙酸盐、乙醇、乳酸盐和琥珀酸盐。L-酒石酸盐和柠檬酸盐发酵生成甲酸盐、乙酸盐和二氧化碳。L-酒石酸盐通过脱水酶途径发酵，葡萄糖通过糖酵解途径发酵。富马酸盐被还原，但硝酸盐、硫酸盐、硫和硫代硫酸盐不被用作末端电子受体。葡萄糖代谢是组成型的，而L-酒石酸盐降解活性是诱导型的。当葡萄糖和L-酒石酸盐同时作为底物时，生长是双相的，首先代谢葡萄糖。在葡萄糖上的生长速率和生长产量高于在L-酒石酸盐上。16Lt1菌株已作为“拟杆菌属”菌种保藏于德国微生物保藏中心，保藏编号为DSM6268。
