Ravot G, Magot M, Ollivier B, Patel B K, Ageron E, Grimont P A, Thomas P, Garcia J L
Laboratoire ORSTOM de Microbiologie Anaérobies, Université de Provence, CESB-ESIL, Marseille, France.
FEMS Microbiol Lett. 1997 Feb 1;147(1):81-8. doi: 10.1111/j.1574-6968.1997.tb10224.x.
A strictly anaerobic, moderately halophilic, Gram-negative, non-motile rod-shaped bacterium was isolated from an oil-well head sample of an offshore Congolese oil field. The strain, designated SEBR 4224T (T = type strain), grew optimally at 42 degrees C and pH 7.0 in a complex medium containing 10% NaCl with a generation time of 2.5 h. Strain SEBR 4224T grew on a range of carbohydrates including fructose, galactose, D-glucose, maltose, D-mannose, D-ribose, sucrose, and trehalose. Yeast extract and/or bio-Trypcase was required for growth on carbohydrates and could not be replaced with amino acids and/or vitamins. The end-products from glucose fermentation were acetate, H2, and CO2. Thiosulfate and elemental sulfur were used as electron acceptors. Thiosulfate improved carbohydrate utilization and biomass yields. The G + C content of the isolate was 34 mol%. Ribosomal 16S rRNA sequence analysis showed that strain SEBR 4224T is a new member of the genus Haloanaerobium. The lack of DNA homology with H. acetoethylicum, its closest relative, as determined by DNA-DNA hybridization supports the designation of strain SEBR 4224T as a new species, Haloanaerobium congolense sp. nov. The type strain is SEBR 4224T (= DSM 11287).
从刚果近海油田的油井口样品中分离出一种严格厌氧、中度嗜盐、革兰氏阴性、无运动性的杆状细菌。该菌株命名为SEBR 4224T（T = 模式菌株），在含有10%氯化钠的复合培养基中，于42℃和pH 7.0条件下生长最佳，代时为2.5小时。SEBR 4224T菌株能利用多种碳水化合物生长，包括果糖、半乳糖、D-葡萄糖、麦芽糖、D-甘露糖、D-核糖、蔗糖和海藻糖。在碳水化合物上生长需要酵母提取物和/或生物胰蛋白胨，不能用氨基酸和/或维生素替代。葡萄糖发酵的终产物是乙酸盐、H2和CO2。硫代硫酸盐和元素硫用作电子受体。硫代硫酸盐提高了碳水化合物的利用率和生物量产量。该分离株的G + C含量为34 mol%。核糖体16S rRNA序列分析表明，SEBR 4224T菌株是嗜盐厌氧菌属的一个新成员。通过DNA-DNA杂交确定，与最接近的亲缘种乙酰乙基嗜盐厌氧菌缺乏DNA同源性，这支持将SEBR 4224T菌株定为一个新物种，即刚果嗜盐厌氧菌（Haloanaerobium congolense sp. nov.）。模式菌株为SEBR 4224T（= DSM 11287）。
