Schulz Patrick, Garcia-Celma Juan J, Fendler Klaus
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max von Laue Str. 3, D-60438 Frankfurt/Main, Germany.
Methods. 2008 Oct;46(2):97-103. doi: 10.1016/j.ymeth.2008.07.002. Epub 2008 Jul 31.
An assay technique for the electrical characterization of electrogenic transport proteins on solid supported membranes is presented. Membrane vesicles, proteoliposomes or membrane fragments containing the transporter are adsorbed to the solid supported membrane and are activated by providing a substrate or a ligand via a rapid solution exchange. This technique opens up new possibilities where conventional electrophysiology fails like transporters or ion channels from bacteria and from intracellular compartments. Its rugged design and potential for automation make it suitable for drug screening.
本文介绍了一种用于在固体支持膜上对电转运蛋白进行电学表征的检测技术。含有转运蛋白的膜囊泡、蛋白脂质体或膜片段被吸附到固体支持膜上，并通过快速溶液交换提供底物或配体来激活。该技术为传统电生理学无法适用的情况开辟了新的可能性，比如来自细菌和细胞内区室的转运蛋白或离子通道。其坚固的设计和自动化潜力使其适用于药物筛选。
