• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转运蛋白溶质载体家族的细胞分析平台概述

An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters.

作者信息

Dvorak Vojtech, Wiedmer Tabea, Ingles-Prieto Alvaro, Altermatt Patrick, Batoulis Helena, Bärenz Felix, Bender Eckhard, Digles Daniela, Dürrenberger Franz, Heitman Laura H, IJzerman Adriaan P, Kell Douglas B, Kickinger Stefanie, Körzö Daniel, Leippe Philipp, Licher Thomas, Manolova Vania, Rizzetto Riccardo, Sassone Francesca, Scarabottolo Lia, Schlessinger Avner, Schneider Vanessa, Sijben Hubert J, Steck Anna-Lena, Sundström Hanna, Tremolada Sara, Wilhelm Maria, Wright Muelas Marina, Zindel Diana, Steppan Claire M, Superti-Furga Giulio

机构信息

CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.

Vifor (International), St. Gallen, Switzerland.

出版信息

Front Pharmacol. 2021 Aug 10;12:722889. doi: 10.3389/fphar.2021.722889. eCollection 2021.

DOI:10.3389/fphar.2021.722889
PMID:34447313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8383457/
Abstract

The solute carrier (SLC) superfamily represents the biggest family of transporters with important roles in health and disease. Despite being attractive and druggable targets, the majority of SLCs remains understudied. One major hurdle in research on SLCs is the lack of tools, such as cell-based assays to investigate their biological role and for drug discovery. Another challenge is the disperse and anecdotal information on assay strategies that are suitable for SLCs. This review provides a comprehensive overview of state-of-the-art cellular assay technologies for SLC research and discusses relevant SLC characteristics enabling the choice of an optimal assay technology. The Innovative Medicines Initiative consortium RESOLUTE intends to accelerate research on SLCs by providing the scientific community with high-quality reagents, assay technologies and data sets, and to ultimately unlock SLCs for drug discovery.

摘要

溶质载体（SLC）超家族是最大的转运蛋白家族，在健康和疾病中发挥着重要作用。尽管是有吸引力且可成药的靶点，但大多数SLC仍未得到充分研究。SLC研究的一个主要障碍是缺乏工具，如用于研究其生物学作用和药物发现的基于细胞的检测方法。另一个挑战是关于适用于SLC的检测策略的信息分散且零散。本综述全面概述了用于SLC研究的最新细胞检测技术，并讨论了相关的SLC特性，以便选择最佳检测技术。创新药物倡议联盟RESOLUTE旨在通过为科学界提供高质量试剂、检测技术和数据集来加速SLC研究，并最终为药物发现解锁SLC。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/577b50013e24/fphar-12-722889-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/11ceb763a550/fphar-12-722889-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/e42182a15a2d/fphar-12-722889-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/f0082bbc9814/fphar-12-722889-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/a97b119f55a6/fphar-12-722889-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/0e8b59986eef/fphar-12-722889-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/1f51eb820948/fphar-12-722889-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/9a2e156d339d/fphar-12-722889-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/eb6abb94d7ae/fphar-12-722889-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/617461afff86/fphar-12-722889-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/1f18ad5b6aae/fphar-12-722889-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/59e1f838ec85/fphar-12-722889-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/577b50013e24/fphar-12-722889-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/11ceb763a550/fphar-12-722889-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/e42182a15a2d/fphar-12-722889-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/f0082bbc9814/fphar-12-722889-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/a97b119f55a6/fphar-12-722889-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/0e8b59986eef/fphar-12-722889-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/1f51eb820948/fphar-12-722889-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/9a2e156d339d/fphar-12-722889-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/eb6abb94d7ae/fphar-12-722889-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/617461afff86/fphar-12-722889-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/1f18ad5b6aae/fphar-12-722889-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/59e1f838ec85/fphar-12-722889-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0fb/8383457/577b50013e24/fphar-12-722889-g012.jpg

相似文献

1
An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters.转运蛋白溶质载体家族的细胞分析平台概述
Front Pharmacol. 2021 Aug 10;12:722889. doi: 10.3389/fphar.2021.722889. eCollection 2021.
2
Advancing drug discovery through assay development: a survey of tool compounds within the human solute carrier superfamily.通过分析方法开发推进药物发现：人类溶质载体超家族内工具化合物的调查
Front Pharmacol. 2024 Jul 9;15:1401599. doi: 10.3389/fphar.2024.1401599. eCollection 2024.
3
Structural and functional annotation of solute carrier transporters: implication for drug discovery.溶质载体转运蛋白的结构与功能注释：对药物发现的启示
Expert Opin Drug Discov. 2023 Jul-Dec;18(10):1099-1115. doi: 10.1080/17460441.2023.2244760. Epub 2023 Aug 10.
4
Establishment of a novel microscale thermophoresis ligand-binding assay for characterization of SLC solute carriers using oligopeptide transporter PepT1 (SLC15 family) as a model system.建立一种新型的微量热泳动配体结合测定法，以寡肽转运体PepT1（SLC15家族）为模型系统来表征SLC溶质载体。
J Pharmacol Toxicol Methods. 2018 Jul-Aug;92:67-76. doi: 10.1016/j.vascn.2018.03.004. Epub 2018 Mar 23.
5
Amino acid transporters within the solute carrier superfamily: Underappreciated proteins and novel opportunities for cancer therapy.溶质载体家族中的氨基酸转运蛋白：被低估的蛋白质和癌症治疗的新机会。
Mol Metab. 2024 Jun;84:101952. doi: 10.1016/j.molmet.2024.101952. Epub 2024 May 3.
6
Data- and knowledge-derived functional landscape of human solute carriers.人类溶质载体的数据与知识衍生功能图谱
Mol Syst Biol. 2025 May 12. doi: 10.1038/s44320-025-00108-2.
7
A rapid and simple non-radioactive assay for measuring uptake by solute carrier transporters.一种用于测量溶质载体转运蛋白摄取的快速简便的非放射性检测方法。
Front Pharmacol. 2024 Apr 24;15:1355507. doi: 10.3389/fphar.2024.1355507. eCollection 2024.
8
Computational Chemistry in Structure-Based Solute Carrier Transporter Drug Design: Recent Advances and Future Perspectives.基于结构的溶质载体转运蛋白药物设计中的计算化学：最新进展和未来展望。
J Chem Inf Model. 2024 Mar 11;64(5):1433-1455. doi: 10.1021/acs.jcim.3c01736. Epub 2024 Jan 31.
9
Targeting SLC transporters: small molecules as modulators and therapeutic opportunities.靶向 SLC 转运体：小分子作为调节剂和治疗机会。
Trends Biochem Sci. 2023 Sep;48(9):801-814. doi: 10.1016/j.tibs.2023.05.011. Epub 2023 Jun 22.
10
Metabolic mapping of the human solute carrier superfamily.人类溶质载体超家族的代谢图谱
Mol Syst Biol. 2025 May 12. doi: 10.1038/s44320-025-00106-4.

引用本文的文献

1
Data- and knowledge-derived functional landscape of human solute carriers.人类溶质载体的数据与知识衍生功能图谱
Mol Syst Biol. 2025 May 12. doi: 10.1038/s44320-025-00108-2.
2
The genetic interaction map of the human solute carrier superfamily.人类溶质载体超家族的遗传相互作用图谱。
Mol Syst Biol. 2025 May 12. doi: 10.1038/s44320-025-00105-5.
3
Metabolic mapping of the human solute carrier superfamily.人类溶质载体超家族的代谢图谱

本文引用的文献

1
In vivo glucose imaging in multiple model organisms with an engineered single-wavelength sensor.在多种模式生物中利用工程化单波长传感器进行活体葡萄糖成像。
Cell Rep. 2021 Jun 22;35(12):109284. doi: 10.1016/j.celrep.2021.109284.
2
A Solid Supported Membrane-Based Technology for Electrophysical Screening of BAT1-Modulating Compounds.基于固体支撑膜的技术用于 BAT1 调节化合物的电泳筛选。
SLAS Discov. 2021 Jul;26(6):783-797. doi: 10.1177/24725552211011180. Epub 2021 May 6.
3
Novel drug targets in 2020.2020年的新型药物靶点。
Mol Syst Biol. 2025 May 12. doi: 10.1038/s44320-025-00106-4.
4
Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches.溶酶体离子通道与转运体：最新发现、治疗潜力及技术方法
Bioelectricity. 2025 Mar 18;7(1):29-57. doi: 10.1089/bioe.2025.0010. eCollection 2025 Mar.
5
The development of a high-throughput acoustic droplet ejection mass-spectrometry assay and a solid-supported membrane (SSM)-based electrophysiological assay to study the pharmacological inhibition of SLC1-A3, -A2 and -A1 in a drug discovery program.在一个药物研发项目中，开发一种高通量声学液滴喷射质谱分析法和一种基于固体支持膜（SSM）的电生理分析法，以研究SLC1 - A3、- A2和 - A1的药理学抑制作用。
Front Pharmacol. 2025 Apr 16;16:1544682. doi: 10.3389/fphar.2025.1544682. eCollection 2025.
6
Neurotrophic factor Neuritin modulates T cell electrical and metabolic state for the balance of tolerance and immunity.神经营养因子神经黏附素调节 T 细胞的电和代谢状态，以平衡耐受和免疫。
Elife. 2024 Nov 20;13:RP96812. doi: 10.7554/eLife.96812.
7
Expression and characterization of pantothenate energy-coupling factor transporters as an anti-infective drug target.泛酸辅酶 A 转运蛋白的表达与鉴定及其作为抗感染药物靶点的研究。
Protein Sci. 2024 Nov;33(11):e5195. doi: 10.1002/pro.5195.
8
Advancing drug discovery through assay development: a survey of tool compounds within the human solute carrier superfamily.通过分析方法开发推进药物发现：人类溶质载体超家族内工具化合物的调查
Front Pharmacol. 2024 Jul 9;15:1401599. doi: 10.3389/fphar.2024.1401599. eCollection 2024.
9
Metabolic gene function discovery platform GeneMAP identifies SLC25A48 as necessary for mitochondrial choline import.代谢基因功能发现平台GeneMAP确定SLC25A48是线粒体胆碱导入所必需的。
Nat Genet. 2024 Aug;56(8):1614-1623. doi: 10.1038/s41588-024-01827-2. Epub 2024 Jul 8.
10
The Competitive Counterflow Assay for Identifying Drugs Transported by Solute Carriers: Principle, Applications, Challenges/Limits, and Perspectives.竞争逆流分析法鉴定溶质载体转运的药物：原理、应用、挑战/限制及展望。
Eur J Drug Metab Pharmacokinet. 2024 Sep;49(5):527-539. doi: 10.1007/s13318-024-00902-7. Epub 2024 Jul 3.
Nat Rev Drug Discov. 2021 May;20(5):333. doi: 10.1038/d41573-021-00057-z.
4
A genetically encoded sensor for measuring serotonin dynamics.用于测量血清素动态变化的基因编码传感器。
Nat Neurosci. 2021 May;24(5):746-752. doi: 10.1038/s41593-021-00823-7. Epub 2021 Apr 5.
5
CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.CRISPR 筛选在生理介质中揭示了人类细胞中条件必需的基因。
Cell Metab. 2021 Jun 1;33(6):1248-1263.e9. doi: 10.1016/j.cmet.2021.02.005. Epub 2021 Mar 1.
6
A study of the dopamine transporter using the TRACT assay, a novel in vitro tool for solute carrier drug discovery.使用新型体外溶质载体药物发现工具——TRACTS 测定法研究多巴胺转运体。
Sci Rep. 2021 Jan 14;11(1):1312. doi: 10.1038/s41598-020-79218-w.
7
The Gene Ontology resource: enriching a GOld mine.基因本体论资源：丰富一个 GOld 矿。
Nucleic Acids Res. 2021 Jan 8;49(D1):D325-D334. doi: 10.1093/nar/gkaa1113.
8
Structural Similarities between Some Common Fluorophores Used in Biology, Marketed Drugs, Endogenous Metabolites, and Natural Products.一些常见的荧光染料在生物学、市售药物、内源性代谢物和天然产物中的结构相似性。
Mar Drugs. 2020 Nov 23;18(11):582. doi: 10.3390/md18110582.
9
UniProt: the universal protein knowledgebase in 2021.UniProt：2021 年的通用蛋白质知识库。
Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489. doi: 10.1093/nar/gkaa1100.
10
MFSD12 mediates the import of cysteine into melanosomes and lysosomes.MFSD12 将半胱氨酸导入黑素体和溶酶体。
Nature. 2020 Dec;588(7839):699-704. doi: 10.1038/s41586-020-2937-x. Epub 2020 Nov 18.