Ciotti Patrizia Maria, Porcu Eleonora, Notarangelo Leonardo, Magrini Otello, Bazzocchi Antonia, Venturoli Stefano
Reproductive Medicine Unit, IVF and Infertility Centre, S.Orsola-Malpighi University Hospital, University of Bologna, Bologna, Italy.
Fertil Steril. 2009 Jun;91(6):2399-407. doi: 10.1016/j.fertnstert.2008.03.013. Epub 2008 Aug 3.
To investigate spindle behavior during and after slow freezing at room temperature (RT) and vitrification at different temperatures.
Randomized, comparative study.
University hospital.
PATIENT(S): Patients undergoing IVF treatment volunteered for the study and donated part of their supernumerary oocytes.
INTERVENTION(S): Metaphase II oocytes were divided into group A: slow freezing RT /thawing RT; group B: vitrification RT/warming RT; group C: vitrification RT/warming 37 degrees C; and group D: vitrification 37 degrees C/warming 37 degrees C. Spindle presence was evaluated at each step of the four procedures and in culture.
MAIN OUTCOME MEASURE(S): Cumulative spindle recovery rate comparing warming phase of the three vitrification groups and culture phase among the four groups.
RESULT(S): During warming, the three vitrification groups showed a significantly fast spindle recovery rate compared to the thawing of the slow freezing group. A progressively significant fast cumulative recovery rate was observed in the three vitrification groups by increasing the number of phases at physiological temperature (hazard rate = 2.68; 95% confidence interval 1.71-4.02).
CONCLUSION(S): The present study demonstrates that spindle recovery is faster in vitrification than in slow freezing. These data support a possible protective effect of vitrification/warming at 37 degrees C on the meiotic spindle structure and, therefore, on the subsequent clinical outcome of the procedure, although comparative clinical studies are needed.
研究室温下慢速冷冻过程中及之后以及在不同温度下玻璃化冷冻时纺锤体的行为。
随机对照研究。
大学医院。
接受体外受精治疗的患者自愿参与本研究并捐赠部分多余的卵母细胞。
将处于减数分裂中期II的卵母细胞分为A组：室温慢速冷冻/室温解冻；B组：室温玻璃化冷冻/室温复温；C组：室温玻璃化冷冻/37℃复温；D组：37℃玻璃化冷冻/37℃复温。在这四种操作的每个步骤以及培养过程中评估纺锤体的存在情况。
比较三个玻璃化冷冻组复温阶段以及四组培养阶段的纺锤体累积恢复率。
在复温过程中，与慢速冷冻组的解冻相比，三个玻璃化冷冻组的纺锤体恢复速度明显更快。通过增加生理温度下的阶段数，在三个玻璃化冷冻组中观察到累积恢复率逐渐显著加快（风险率=2.68；95%置信区间1.71 - 4.02）。
本研究表明，玻璃化冷冻时纺锤体的恢复比慢速冷冻更快。这些数据支持在37℃下进行玻璃化冷冻/复温对减数分裂纺锤体结构可能具有保护作用，因此对该操作随后的临床结局也可能有保护作用，不过还需要进行比较性临床研究。
