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生物材料的冷冻保存:应用与经济前景

Cryopreservation of biological materials: applications and economic perspectives.

作者信息

Aarattuthodi Suja, Kang David, Gupta Sanjay Kumar, Chen Paula, Redel Bethany, Matuha Moureen, Mohammed Haitham, Sinha Amit Kumar

机构信息

Plant Genetics Research Unit, United States Department of Agriculture - Agricultural Research Service, Columbia, MO, 65211, USA.

Biological Control of Insects Research Laboratory, United States Department of Agriculture - Agricultural Research Service, Columbia, MO, 65211, USA.

出版信息

In Vitro Cell Dev Biol Anim. 2025 Apr 23. doi: 10.1007/s11626-025-01027-0.

Abstract

Cryopreservation is a transformative technology that allows for the long-term storage of biological materials by cooling them to extremely low temperatures at which metabolic and biochemical processes are effectively slowed or halted. Cryopreservation utilizes various techniques to minimize ice crystal formation and cellular damage during freezing and thawing processes. This technology has broad applications in the fields of medicine, agriculture, and conservation, spanning across stem cell research, reproductive and regenerative medicine, organ transplantation, and cell-based therapies, each with significant economic implications. While current techniques and their associated costs present certain challenges, ongoing research advancements related to cryoprotectants, cooling methods, and automation promise to enhance efficiency and accessibility, potentially broadening the technology's impact across various sectors. This review focuses on the applications of cryopreservation, research advancements, and economic implications, emphasizing the importance of continued research to overcome the current limitations.

摘要

冷冻保存是一项变革性技术,它通过将生物材料冷却至极低温度,使代谢和生化过程有效减缓或停止,从而实现生物材料的长期储存。冷冻保存在冷冻和解冻过程中运用各种技术,以尽量减少冰晶形成和细胞损伤。这项技术在医学、农业和保护领域有着广泛应用,涵盖干细胞研究、生殖与再生医学、器官移植以及细胞疗法等,每个领域都具有重大经济影响。虽然当前技术及其相关成本带来了一定挑战,但与冷冻保护剂、冷却方法和自动化相关的正在进行的研究进展有望提高效率和可及性,可能会扩大该技术在各个领域的影响。本综述重点关注冷冻保存的应用、研究进展和经济影响,强调持续研究以克服当前局限性的重要性。

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