Wu Xi-li, Sun Wan-sen, Zhang Wang-gang, Wang Zhu
Department of Integrated Traditional Chinese and Western Medicine, Shaanxi Provincial Key TCM, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jul;28(7):1198-201.
To investigate the effects of Tongluo Recipe on the expression of collagen IV (Col IV), fibronectin (FN), laminin (LN), transforming growth factor-beta1 (TGF-beta1) in rat renal tissues and explore the mechanism underlying these effects in rats with glomerular sclerosis.
The pathological changes in the renal tissues of rats with glomerular sclerosis were observed microscopically, and the expressions of Col IV, FN, LN, and TGF-beta1 were detected using immunohistochemical staining and image analysis system.
Tongluo Recipe significantly decreased the expressions of Col IV, FN, LN and TGF-beta1 in the renal tissue of rats with glomerular sclerosis (P<0.05 or P<0.01) and obviously alleviated the renal pathologies (P<0.01).
The therapeutic effects of Tongluo Recipe are probably mediated by lowered expressions of Col IV, FN, LN and TGF-beta1.
探讨通络方对肾小球硬化大鼠肾组织中Ⅳ型胶原（Col Ⅳ）、纤连蛋白（FN）、层粘连蛋白（LN）、转化生长因子-β1（TGF-β1）表达的影响，并探讨其作用机制。
光镜下观察肾小球硬化大鼠肾组织的病理变化，采用免疫组化染色及图像分析系统检测Col Ⅳ、FN、LN及TGF-β1的表达。
通络方显著降低肾小球硬化大鼠肾组织中Col Ⅳ、FN、LN及TGF-β1的表达（P<0.05或P<0.01），明显减轻肾脏病理改变（P<0.01）。
通络方的治疗作用可能是通过降低Col Ⅳ、FN、LN及TGF-β1的表达来实现的。
