Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
Phytomedicine. 2013 Aug 15;20(11):1023-9. doi: 10.1016/j.phymed.2013.04.007. Epub 2013 Jun 5.
To investigate the effect of catalpol on diabetic nephropathy in rats.
Male Sprague-Dawley rats were randomly divided into two groups and fed with normal pallet diet (NPD) or high-fat diet (HFD) for 4 weeks respectively. Then the HFD-fed rats were injected with 35 mg/kg streptozotocin (STZ) for establishing diabetic model. The diabetic rats were randomly divided into five groups: model group, model plus catalpol 30, 60, 120 mg/kg groups and model plus metformin 200 mg/kg group. The NPD-fed rats were randomly divided into two groups: normal control group and normal plus catalpol 60 mg/kg control group. After administration for 10 weeks, random blood glucose (RBG), glycated serum protein (GSP), 24h urinary protein excretion (UPE), serum creatinine (Scr), blood urea nitrogen (BUN), and kidney weight index (KWI) were determined. The kidney pathological changes were evaluated by periodic acid-Schiff (PAS) staining. The concentrations of angiotensin II (Ang II), transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), fibronectin (FN), collagen type IV (Col IV) in renal cortex were determined. Real time RT-PCR was used to detect the mRNA expressions of TGF-β1 and CTGF.
Catalpol could significantly reduce the KWI, improve the kidney function and pathological change, decrease the tissue level of Ang II, TGF-β1, CTGF, FN, Col IV. Catalpol could also down regulate the mRNA expressions of TGF-β1 and CTGF in renal cortex.
Catalpol may have beneficial effects against diabetic nephropathy. The mechanisms may be related to reducing the extracellular matrix accumulation by restraining the expression of TGF-β1, CTGF and Ang II.
研究梓醇对糖尿病肾病大鼠的作用。
雄性 Sprague-Dawley 大鼠随机分为两组，分别给予正常饲料（NPD）或高脂饲料（HFD）喂养 4 周。然后，用 35mg/kg 链脲佐菌素（STZ）给 HFD 喂养的大鼠注射以建立糖尿病模型。将糖尿病大鼠随机分为五组：模型组、模型加梓醇 30、60、120mg/kg 组和模型加二甲双胍 200mg/kg 组。给予 NPD 喂养的大鼠随机分为两组：正常对照组和正常加梓醇 60mg/kg 对照组。给药 10 周后，测定随机血糖（RBG）、糖化血清蛋白（GSP）、24h 尿蛋白排泄（UPE）、血清肌酐（Scr）、血尿素氮（BUN）和肾重指数（KWI）。用过碘酸-Schiff（PAS）染色评价肾脏病理变化。测定肾皮质中血管紧张素 II（Ang II）、转化生长因子-β1（TGF-β1）、结缔组织生长因子（CTGF）、纤维连接蛋白（FN）、IV 型胶原（Col IV）的浓度。实时 RT-PCR 用于检测 TGF-β1 和 CTGF 的 mRNA 表达。
梓醇可显著降低 KWI，改善肾脏功能和病理变化，降低组织中 Ang II、TGF-β1、CTGF、FN、Col IV 水平。梓醇还可以下调肾皮质中 TGF-β1 和 CTGF 的 mRNA 表达。
梓醇可能对糖尿病肾病有有益作用。其机制可能与通过抑制 TGF-β1、CTGF 和 Ang II 的表达减少细胞外基质积聚有关。
