Bessler W G, Beck P, Konetznick U, Loleit M, Sedelmeier E, Hoffmann P, Strecker M, Stöcklin S
Institut für Immunbiologie der Universität, Freiburg/Brsg, Fed. Rep. of Germany.
Arzneimittelforschung. 1991 Mar;41(3):274-9.
The immunogenic and immunomodulatory properties of a lysed fraction from selected E. coli strains (OM-89, Uro-Vaxom) were determined in vivo and in vitro. It could be demonstrated that OM-89 constitutes an active immunogen in mice. Maximum OM-89-specific antibody titers were obtained after 4-5 i.p. immunizations; the titers could be further enhanced by the simultaneous injection of lipopeptide adjuvants. It was shown by ELISA that the antibodies obtained bound to the bacterial strains used for the preparation of the OM-89 extract. Immunogenicity was observed both after intraperitoneal and oral application of the extract. Besides being active as an immunogen. OM-89 was able to act in vitro as a polyclonal lymphocyte activator, as determined in splenocyte cultures of different inbred murine strains, and in cultures of human peripheral blood lymphocytes. Our results show that the B lymphocyte stimulating activity of the bacterial extract OM-89 was comparable to that of lipopeptide adjuvants. In conclusion, the bacterial extract both an active immunogen in vivo, and a polyclonal B cell activator in vitro. These findings may be of importance for the understanding of the therapeutic effect of OM-89.
对选定大肠杆菌菌株（OM - 89、Uro - Vaxom）裂解组分的免疫原性和免疫调节特性进行了体内和体外测定。结果表明，OM - 89在小鼠体内构成一种活性免疫原。经4 - 5次腹腔注射免疫后可获得最高的OM - 89特异性抗体滴度；同时注射脂肽佐剂可进一步提高滴度。ELISA检测显示，所获得的抗体与用于制备OM - 89提取物的细菌菌株结合。提取物经腹腔注射和口服后均观察到免疫原性。除了作为一种活性免疫原外，如在不同近交系小鼠品系的脾细胞培养物以及人外周血淋巴细胞培养物中所测定的那样，OM - 89在体外还能够作为一种多克隆淋巴细胞激活剂发挥作用。我们的结果表明，细菌提取物OM - 89的B淋巴细胞刺激活性与脂肽佐剂相当。总之，该细菌提取物在体内是一种活性免疫原，在体外是一种多克隆B细胞激活剂。这些发现对于理解OM - 89的治疗效果可能具有重要意义。
