Sedelmeier E A, Bessler W G
Institut für Immunbiologie der Universität, Freiburg, Germany.
Immunopharmacology. 1995 Feb;29(1):29-36. doi: 10.1016/0162-3109(95)00041-q.
The bacterial extract OM-89 is used for the treatment of recurrent infections of the urinary tract. It consists of immunostimulating components derived from 18 Escherichia coli bacterial strains and constitutes a non-specific leukocyte activator. We here could demonstrate for the first time that, in a long-term immunization protocol, OM-89 acts as an effective immunogen in mice. After multiple parenteral applications of the extract, OM-89-specific antisera were obtained; the maximum serum antibody content was found after 6-9 immunizations. The antibodies induced were mainly of the IgG isotype, and a weak increase of IgM was observed. The sera also bound to the bacterial strains used for the preparation of the extract. Moreover, we could show that defined bacterial cell-wall components like protein I (porin), lipopeptide and murein were recognized. Our findings on the immunogenicity of OM-89 might partially explain the therapeutic effect of the extract as shown in clinical studies.
细菌提取物OM-89用于治疗复发性尿路感染。它由源自18种大肠杆菌菌株的免疫刺激成分组成，是一种非特异性白细胞激活剂。我们在此首次证明，在长期免疫方案中，OM-89在小鼠中作为一种有效的免疫原起作用。多次肠胃外应用该提取物后，获得了OM-89特异性抗血清；在6至9次免疫后发现血清抗体含量最高。诱导产生的抗体主要为IgG同种型，同时观察到IgM有微弱增加。这些血清也与用于制备提取物的细菌菌株结合。此外，我们能够证明特定的细菌细胞壁成分如蛋白I（孔蛋白）、脂肽和胞壁质被识别。我们关于OM-89免疫原性的发现可能部分解释了临床研究中所示提取物的治疗效果。
