Renal pathomorphology and histochemistry in dog after hemodilution as well as after administration of full blood in extracorporeal circulation in dog.

Dog kidneys were studied morphologically and histochemically after hemodilution to a 10--20% hematocrict value and transfusion of whole blood under conditions of extracorporeal circulation ensuring constant (linear) flow. Marked interstitial edema in the dog kidneys after hemodilution was the most pronounced alteration, attributed to disorders in the capillary circulation depending upon the apparatus employed. This conclusion was supported by similar changes in animals subjected to various degrees of hemodilution. Widening of the glomerular space and compression of renal tubules were the direct consequences of interstitial edema. Histochemical reactions revealed damage of the cell membranes. Swelling of the ergastoplasm and lysosomes was interpreted as secondary to disorders in circulation of tissue fluid. The least pronounced changes concerned the mitochondria. After extracorporeal circulation with whole blood, thrombi and fat emboli were observed in the blood vessels of the renal glomeruli, attributable to damage of blood in the extracorporeal circulation apparatus.