Sorokin D Y, van den Bosch P L F, Abbas B, Janssen A J H, Muyzer G
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
Appl Microbiol Biotechnol. 2008 Oct;80(6):965-75. doi: 10.1007/s00253-008-1598-8. Epub 2008 Aug 2.
Thiopaq biotechnology for partial sulfide oxidation to elemental sulfur is an efficient way to remove H(2)S from biogases. However, its application for high-pressure natural gas desulfurization needs upgrading. Particularly, an increase in alkalinity of the scrubbing liquid is required. Therefore, the feasibility of sulfide oxidation into elemental sulfur under oxygen limitation was tested at extremely haloalkaline conditions in lab-scale bioreactors using mix sediments from hypersaline soda lakes as inoculum. The microbiological analysis, both culture dependent and independent, of the successfully operating bioreactors revealed a domination of obligately chemolithoautotrophic and extremely haloalkaliphilic sulfur-oxidizing bacteria belonging to the genus Thioalkalivibrio. Two subgroups were recognized among the isolates. The subgroup enriched from the reactors operating at pH 10 clustered with Thioalkalivibrio jannaschii-Thioalkalivibrio versutus core group of the genus Thioalkalivibrio. Another subgroup, obtained mostly with sulfide as substrate and at lower pH, belonged to the cluster of facultatively alkaliphilic Thioalkalivibrio halophilus. Overall, the results clearly indicate a large potential of the genus Thiolalkalivibrio to efficiently oxidize sulfide at extremely haloalkaline conditions, which makes it suitable for application in the natural gas desulfurization.
Thiopaq生物技术可将部分硫化物氧化为元素硫，是从生物气中去除H₂S的有效方法。然而，其在高压天然气脱硫中的应用需要改进。特别是，需要提高洗涤液的碱度。因此，在实验室规模的生物反应器中，使用高盐苏打湖的混合沉积物作为接种物，在极端盐碱条件下测试了在氧气限制下将硫化物氧化为元素硫的可行性。对成功运行的生物反应器进行的依赖培养和不依赖培养的微生物分析表明，属于硫碱弧菌属的专性化能自养和极端嗜盐碱硫氧化细菌占主导地位。在分离物中识别出两个亚群。从pH值为10的反应器中富集的亚群与硫碱弧菌属的詹氏硫碱弧菌-韦氏硫碱弧菌核心群聚集在一起。另一个亚群主要以硫化物为底物，在较低pH值下获得，属于兼性嗜碱嗜盐硫碱弧菌的聚类。总体而言，结果清楚地表明硫碱弧菌属在极端盐碱条件下有效氧化硫化物的巨大潜力，这使其适用于天然气脱硫。
