Lorenzo P, de Silóniz M I, Perera J
Departamento de Bioquímica y Biología Molecular, I, Facultades de Ciencias Biológicas y Químicas, Universidad Complutense de Madrid, Spain.
Microbiologia. 1991 Jun;7(1):53-6.
Different quantitative methods to evaluate cell concentrations in acidophilic iron- and sulphur-oxidizing microorganisms liquid cultures have been compared with the "most probable number of viable germs" procedure. Plating and colony counting on recently developed solid media give good results when used with pure bacterial cultures, but plating efficiency decreases with natural mixed cultures.
已将不同的定量方法用于评估嗜酸铁氧化和硫氧化微生物液体培养物中的细胞浓度,并与“活菌最可能数”程序进行了比较。在最近开发的固体培养基上进行平板接种和菌落计数,用于纯细菌培养时效果良好,但用于天然混合培养时平板接种效率会降低。