Gupta Pankaj, Gaur Vineet, Salunke Dinakar M
National Institute of Immunology, New Delhi 110067, India.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):733-6. doi: 10.1107/S1744309108021970. Epub 2008 Jul 26.
Lens culinaris (lentil) is a widely consumed high-protein-content leguminous crop. A 2S albumin protein (26.5 kDa) has been identified using NH(2)-terminal sequencing from a 90% ammonium sulfate saturation fraction of total L. culinaris seed protein extract. The NH(2)-terminal sequence shows very high homology to PA2, an allergy-related protein from Pisum sativum. The 2S albumin protein was purified using a combination of size-exclusion and ion-exchange chromatography. Crystals of the 2S seed albumin obtained using the hanging-drop vapour-diffusion method diffracted to 2.5 A resolution and were indexed in space group P4(1) (or P4(3)), with unit-cell parameters a = b = 78.6, c = 135.2 A.
兵豆是一种被广泛食用的、高蛋白含量的豆科作物。通过对兵豆种子总蛋白提取物90%硫酸铵饱和度级分进行N端测序，已鉴定出一种2S清蛋白（26.5 kDa）。该N端序列与来自豌豆的一种过敏相关蛋白PA2具有很高的同源性。采用尺寸排阻色谱和离子交换色谱相结合的方法对2S清蛋白进行了纯化。利用悬滴气相扩散法获得的2S种子清蛋白晶体衍射分辨率为2.5 Å，并确定其空间群为P4(1)（或P4(3)），晶胞参数a = b = 78.6，c = 135.2 Å。
