小扁豆2S白蛋白种子蛋白的纯化、鉴定及初步晶体学研究
Purification, identification and preliminary crystallographic studies of a 2S albumin seed protein from Lens culinaris.
作者信息
Gupta Pankaj, Gaur Vineet, Salunke Dinakar M
机构信息
National Institute of Immunology, New Delhi 110067, India.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):733-6. doi: 10.1107/S1744309108021970. Epub 2008 Jul 26.
Abstract
Lens culinaris (lentil) is a widely consumed high-protein-content leguminous crop. A 2S albumin protein (26.5 kDa) has been identified using NH(2)-terminal sequencing from a 90% ammonium sulfate saturation fraction of total L. culinaris seed protein extract. The NH(2)-terminal sequence shows very high homology to PA2, an allergy-related protein from Pisum sativum. The 2S albumin protein was purified using a combination of size-exclusion and ion-exchange chromatography. Crystals of the 2S seed albumin obtained using the hanging-drop vapour-diffusion method diffracted to 2.5 A resolution and were indexed in space group P4(1) (or P4(3)), with unit-cell parameters a = b = 78.6, c = 135.2 A.
摘要
兵豆是一种被广泛食用的、高蛋白含量的豆科作物。通过对兵豆种子总蛋白提取物90%硫酸铵饱和度级分进行N端测序，已鉴定出一种2S清蛋白（26.5 kDa）。该N端序列与来自豌豆的一种过敏相关蛋白PA2具有很高的同源性。采用尺寸排阻色谱和离子交换色谱相结合的方法对2S清蛋白进行了纯化。利用悬滴气相扩散法获得的2S种子清蛋白晶体衍射分辨率为2.5 Å，并确定其空间群为P4(1)（或P4(3)），晶胞参数a = b = 78.6，c = 135.2 Å。
相似文献
1
Purification, identification and preliminary crystallographic studies of a 2S albumin seed protein from Lens culinaris.小扁豆2S白蛋白种子蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):733-6. doi: 10.1107/S1744309108021970. Epub 2008 Jul 26.
2
Purification, identification and preliminary crystallographic studies of an allergenic protein from Lathyrus sativus.来自草香豌豆的一种变应原蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt 9):869-72. doi: 10.1107/S1744309106028077. Epub 2006 Aug 11.
3
Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena.茄子中一种致敏蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):221-5. doi: 10.1107/S2053230X15000734. Epub 2015 Jan 28.
4
Purification, identification and preliminary crystallographic characterization of a novel seed protein from Vigna unguiculata.来自豇豆的一种新型种子蛋白的纯化、鉴定及初步晶体学表征
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2100-3. doi: 10.1107/S0907444904023868. Epub 2004 Oct 20.
5
Biochemical and functional characterization of an albumin protein belonging to the hemopexin superfamily from Lens culinaris seeds.菜豆种子属血红素结合蛋白超家族的白蛋白的生化和功能特征。
J Agric Food Chem. 2011 Sep 14;59(17):9637-44. doi: 10.1021/jf202026d. Epub 2011 Aug 18.
6
Purification, characterization and preliminary crystallographic studies of a PR-10 protein from Pachyrrhizus erosus seeds.
Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2165-7. doi: 10.1107/s0907444902016062. Epub 2002 Nov 23.
7
Purification, crystallization and preliminary crystallographic studies of a Kunitz-type proteinase inhibitor from tamarind (Tamarindus indica) seeds.罗望子（罗望子属）种子中一种Kunitz型蛋白酶抑制剂的纯化、结晶及初步晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt 7):736-8. doi: 10.1107/S1744309109023495. Epub 2009 Jun 30.
8
Characterisation of 2S albumin with nutritionally balanced aminoacid composition from the seeds of Chenopodium album and its antigenic homology with seed proteins of some Chenopodiaceae and Amaranthaceae species.藜种子中具有营养均衡氨基酸组成的2S白蛋白的表征及其与一些藜科和苋科物种种子蛋白的抗原同源性。
Biochem Mol Biol Int. 1993 May;30(1):149-57.
9
Purification, characterization and preliminary crystallographic studies of a cysteine protease from Pachyrrhizus erosus seeds.
Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):187-9. doi: 10.1107/s0907444903025939. Epub 2003 Dec 18.
10
Crystallization and preliminary X-ray diffraction analysis of a novel trypsin inhibitor from seeds of Copaifera langsdorffii.来自巴西香脂树种子的一种新型胰蛋白酶抑制剂的结晶及初步X射线衍射分析。
Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1316-8. doi: 10.1107/s0907444901011131. Epub 2001 Aug 23.
引用本文的文献
1
Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena.茄子中一种致敏蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):221-5. doi: 10.1107/S2053230X15000734. Epub 2015 Jan 28.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis.巴旦木（扁桃）中的一种变应原蛋白——苦杏仁球蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):32-5. doi: 10.1107/S1744309107064615. Epub 2007 Dec 20.
3
Purification, identification and preliminary crystallographic studies of an allergenic protein from Lathyrus sativus.来自草香豌豆的一种变应原蛋白的纯化、鉴定及初步晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt 9):869-72. doi: 10.1107/S1744309106028077. Epub 2006 Aug 11.
4
Crystallization and preliminary structure determination of the plant food allergen Pru av 2.植物食物过敏原Pru av 2的结晶及初步结构测定
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt 2):186-8. doi: 10.1107/S1744309104033822. Epub 2005 Jan 8.
5
Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions.2S白蛋白型花生过敏原的结构与稳定性：对花生过敏反应严重程度的影响
Biochem J. 2006 May 1;395(3):463-72. doi: 10.1042/BJ20051728.
6
Prevalence of food allergy: an overview.食物过敏的患病率：概述
Proc Nutr Soc. 2005 Nov;64(4):413-7. doi: 10.1079/pns2005458.
7
Complete assignment and secondary structure of the Brazil nut allergen Ber e 1.巴西坚果过敏原Ber e 1的完整一级结构和二级结构。
J Biomol NMR. 2005 Aug;32(4):336. doi: 10.1007/s10858-005-8875-y.
8
Gastrointestinal food allergy: new insights into pathophysiology and clinical perspectives.胃肠道食物过敏：病理生理学与临床前景的新见解
Gastroenterology. 2005 Apr;128(4):1089-113. doi: 10.1053/j.gastro.2004.08.015.
9
Molecular properties of food allergens.食物过敏原的分子特性
J Allergy Clin Immunol. 2005 Jan;115(1):14-23; quiz 24. doi: 10.1016/j.jaci.2004.10.022.
10
Purification, identification and preliminary crystallographic characterization of a novel seed protein from Vigna unguiculata.来自豇豆的一种新型种子蛋白的纯化、鉴定及初步晶体学表征
Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2100-3. doi: 10.1107/S0907444904023868. Epub 2004 Oct 20.
Zotero 插件