Jain Abha, Salunke Dinakar Masanu
Regional Centre for Biotechnology, 180 Udyog Vihar Phase 1, Gurgaon, Haryana 122 016, India.
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):221-5. doi: 10.1107/S2053230X15000734. Epub 2015 Jan 28.
Solanum melongena (eggplant), a member of the Solanaceae family, is a widely cultivated vegetable crop and is commonly used as a food throughout the world. Allergic reactions caused by members of this family are well known. However, mechanistic analyses to understand their molecular basis have not been adequately explored. In order to address this issue, the 7S vicilin protein (SM80.1) of size 45 kDa was purified from seeds of S. melongena by ammonium sulfate fractionation and size-exclusion chromatography. Significant homology of SM80.1 to an allergy-related protein from S. lycopersicum was identified through a BLAST search. Crystallization attempts with purified protein using the hanging-drop vapour-diffusion method led to hexagonal-shaped crystals. The crystals diffracted to 2.21 Å resolution and belonged to space group P6322, with unit-cell parameters a = 117.9, c = 123.5 Å.
茄子（Solanum melongena）是茄科植物的一员，是一种广泛种植的蔬菜作物，在全世界普遍被用作食物。由该科植物引起的过敏反应是众所周知的。然而，尚未充分探索了解其分子基础的机制分析。为了解决这个问题，通过硫酸铵分级分离和尺寸排阻色谱法从茄子种子中纯化出了大小为45 kDa的7S豌豆球蛋白（SM80.1）。通过BLAST搜索确定了SM80.1与来自番茄（S. lycopersicum）的一种过敏相关蛋白具有显著同源性。使用悬滴气相扩散法对纯化蛋白进行结晶尝试，得到了六边形晶体。这些晶体的衍射分辨率为2.21 Å，属于空间群P6322，晶胞参数a = 117.9，c = 123.5 Å。
