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本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Biochemical and genetic analysis of the gamma-resorcylate (2,6-dihydroxybenzoate) catabolic pathway in Rhizobium sp. strain MTP-10005: identification and functional analysis of its gene cluster.根瘤菌属菌株MTP - 10005中γ - 间苯二酚（2,6 - 二羟基苯甲酸）分解代谢途径的生化与遗传分析：其基因簇的鉴定与功能分析
J Bacteriol. 2007 Mar;189(5):1573-81. doi: 10.1128/JB.01675-06. Epub 2006 Dec 8.
3
Crystal structures of nonoxidative zinc-dependent 2,6-dihydroxybenzoate (gamma-resorcylate) decarboxylase from Rhizobium sp. strain MTP-10005.来自根瘤菌属菌株MTP - 10005的非氧化型锌依赖性2,6 - 二羟基苯甲酸（γ - 间苯二酚酸）脱羧酶的晶体结构。
J Biol Chem. 2006 Nov 10;281(45):34365-73. doi: 10.1074/jbc.M607270200. Epub 2006 Sep 8.
4
Thermophilic, reversible gamma-resorcylate decarboxylase from Rhizobium sp. strain MTP-10005: purification, molecular characterization, and expression.来自根瘤菌属菌株MTP-10005的嗜热可逆γ-间苯二酚羧化酶：纯化、分子特性及表达
J Bacteriol. 2004 Oct;186(20):6855-63. doi: 10.1128/JB.186.20.6855-6863.2004.
5
Does lowering glutamine synthetase activity in nodules modify nitrogen metabolism and growth of Lotus japonicus?降低根瘤中谷氨酰胺合成酶的活性是否会改变日本百脉根的氮代谢和生长？
Plant Physiol. 2003 Sep;133(1):253-62. doi: 10.1104/pp.102.016766.
6
Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13.编码假单胞菌属菌株B13马来酰乙酸还原酶的clcE基因的克隆、特性鉴定及序列分析
J Bacteriol. 1997 Jun;179(11):3801-3. doi: 10.1128/jb.179.11.3801-3803.1997.
7
Degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9.假单胞菌属BN9对2,4-二羟基苯甲酸的降解作用
FEMS Microbiol Lett. 1993 Apr 1;108(2):219-24. doi: 10.1111/j.1574-6968.1993.tb06102.x.
8
Degradation of chloroaromatics: purification and characterization of maleylacetate reductase from Pseudomonas sp. strain B13.氯代芳烃的降解：来自假单胞菌属B13菌株的马来酰乙酸还原酶的纯化与表征
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9
Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4).从嗜碱假单胞菌JMP134(pJP4)中纯化和鉴定马来酸乙酸还原酶
J Bacteriol. 1993 Nov;175(21):6745-54. doi: 10.1128/jb.175.21.6745-6754.1993.
10
Evidence of two pathways for the metabolism of phenol by Aspergillus fumigatus.烟曲霉代谢苯酚的两条途径的证据。
Arch Microbiol. 1995 Mar;163(3):176-81. doi: 10.1007/BF00305350.

来自根瘤菌属菌株MTP - 10005的马来酰乙酸还原酶的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of maleylacetate reductase from Rhizobium sp. strain MTP-10005.

作者信息

Fujii Tomomi, Goda Yuko, Yoshida Masahiro, Oikawa Tadao, Hata Yasuo

机构信息

Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):737-9. doi: 10.1107/S1744309108022537. Epub 2008 Jul 31.

DOI:10.1107/S1744309108022537
PMID:18678945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2494958/
Abstract

Maleylacetate reductase (EC 1.3.1.32), which catalyzes the reduction of maleylacetate to 3-oxoadipate, plays an important role in the aerobic microbial catabolism of resorcinol. The enzyme has been crystallized at 293 K by the sitting-drop vapour-diffusion method supplemented with a microseeding technique, using ammonium sulfate as the precipitating agent. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 56.85, b = 121.13, c = 94.09 A, beta = 101.48 degrees , and contained one dimeric molecule in the asymmetric unit. It diffracted to 1.79 A resolution.

摘要

马来酰乙酸还原酶（EC 1.3.1.32）催化马来酰乙酸还原为3-氧代己二酸，在间苯二酚的需氧微生物分解代谢中起重要作用。该酶通过坐滴气相扩散法并辅以微种子技术，以硫酸铵作为沉淀剂，在293 K下结晶。晶体属于单斜空间群C2，晶胞参数a = 56.85，b = 121.13，c = 94.09 Å，β = 101.48°，不对称单元中包含一个二聚体分子。其衍射分辨率达到1.79 Å。