Stolz A, Knackmuss H J
Institut für Mikrobiologie, Universität Stuttgart, FRG.
FEMS Microbiol Lett. 1993 Apr 1;108(2):219-24. doi: 10.1111/j.1574-6968.1993.tb06102.x.
The aerobic degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9 was studied. Intact cells of Pseudomonas sp. BN9 grown with 2,4-dihydroxybenzoate oxidized 2,4-dihydroxybenzoate but not salicylate. Cell-free extracts of Pseudomonas sp. BN9 converted 2,4-dihydroxybenzoate after the addition of NAD(P)H. A partially purified protein fraction converted 2,4-dihydroxybenzoate with NADH to 1,2,4-trihydroxybenzene. 1,2,4-Trihydroxybenzene was converted by a 1,2-dioxygenase to maleylpyruvate, which was reduced by a NADH-dependent enzyme to 3-oxoadipate. 2,4-Dihydroxybenzoate 1-monooxygenase, 1,2,4-trihydroxybenzene 1,2-dioxygenase and maleylpyruvate reductase were induced in Pseudomonas sp. BN9 after growth with 2,4-dihydroxybenzoate.
研究了假单胞菌属BN9对2,4 - 二羟基苯甲酸的好氧降解。以2,4 - 二羟基苯甲酸生长的假单胞菌属BN9完整细胞可氧化2,4 - 二羟基苯甲酸，但不能氧化水杨酸。在添加NAD(P)H后，假单胞菌属BN9的无细胞提取物可转化2,4 - 二羟基苯甲酸。一个部分纯化的蛋白质组分可利用NADH将2,4 - 二羟基苯甲酸转化为1,2,4 - 三羟基苯。1,2,4 - 三羟基苯被一种1,2 - 双加氧酶转化为马来酰丙酮酸，后者被一种依赖NADH的酶还原为3 - 氧代己二酸。在以2,4 - 二羟基苯甲酸生长后，假单胞菌属BN9中诱导产生了2,4 - 二羟基苯甲酸1 - 单加氧酶、1,2,4 - 三羟基苯1,2 - 双加氧酶和马来酰丙酮酸还原酶。
