Liu Qi, Gao Yang, Yang Weili, Zhou Huihao, Gao Yongxiang, Zhang Xiao, Teng Maikun, Niu Liwen
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):743-5. doi: 10.1107/S1744309108020241. Epub 2008 Jul 31.
Transfer RNA (tRNA) (m(7)G46) methyltransferase (TrmB) belongs to the Rossmann-fold methyltransferase (RFM) family and uses S-adenosyl-L-methionine (SAM) as the methyl-group donor to catalyze the formation of N(7)-methylguanosine (m(7)G) at position 46 in the variable loop of tRNAs. After attempts to crystallize full-length Escherichia coli TrmB (EcTrmB) failed, a truncated protein lacking the first 32 residues of the N-terminus but with an additional His(6) tag at the C-terminus was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 (PEG 3350) as precipitant at 283 K. An X-ray diffraction data set was collected using a single flash-cooled crystal that belonged to space group P2(1).
转运RNA（tRNA）（m⁷G46）甲基转移酶（TrmB）属于罗斯曼折叠甲基转移酶（RFM）家族，它以S-腺苷-L-甲硫氨酸（SAM）作为甲基供体，催化tRNA可变环第46位的N⁷-甲基鸟苷（m⁷G）的形成。在尝试结晶全长大肠杆菌TrmB（EcTrmB）失败后，通过悬滴气相扩散法，以聚乙二醇3350（PEG 3350）作为沉淀剂，在283 K下结晶出一种截短蛋白，该蛋白缺少N端的前32个残基，但在C端带有一个额外的His⁶标签。使用属于空间群P2₁的单个快速冷却晶体收集了X射线衍射数据集。
