嗜热拟青霉1,3-1,4-β-葡聚糖酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila.
作者信息
Yang Shaoqing, Wang Yaxi, Jiang Zhengqiang, Hua Chengwei
机构信息
Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):754-6. doi: 10.1107/S1744309108021064. Epub 2008 Jul 31.
Abstract
In this study, the crystallization and preliminary X-ray analysis of a thermostable 1,3-1,4-beta-glucanase produced by Paecilomyces thermophila is described. The purified 1,3-1,4-beta-glucanase was crystallized using the hanging-drop vapour-diffusion method. The crystal belongs to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 154.54, c = 87.62 A. X-ray diffraction data were collected to a resolution of 2.54 A and gave a data set with an overall R(merge) of 7.3% and a completeness of 94.6%. The Matthews coefficient (V(M)) and the solvent content are 2.38 A(3) Da(-1) and 48%, respectively.
摘要
在本研究中，描述了嗜热拟青霉产生的一种耐热1,3-1,4-β-葡聚糖酶的结晶及初步X射线分析。纯化后的1,3-1,4-β-葡聚糖酶采用悬滴气相扩散法进行结晶。晶体属于六方空间群P6(3)22，晶胞参数a = b = 154.54，c = 87.62 Å。X射线衍射数据收集至2.54 Å分辨率，得到的数据集整体R(merge)为7.3%，完整性为94.6%。马修斯系数（V(M)）和溶剂含量分别为2.38 Å(3) Da(-1)和48%。
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