Matrawy Amira A, Khalil Ahmed I, Marey Heba S, Embaby Amira M
Environmental Studies Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt.
Biotechnology Department, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt.
J Fungi (Basel). 2021 Aug 27;7(9):696. doi: 10.3390/jof7090696.
The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett-Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(/)), yeast extract concentration (%(/)), and initial pH of the production medium imposed significant effects ( ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (/), 5.1, and 0.098% (/), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase.
本研究首次强调了利用体积庞大且难降解的木质纤维素副产品小麦秸秆（WS），通过本地来源的新型菌株A3 DSM105774来提高增值木聚糖酶产量的价值。采用三步统计和顺序方法，即一次一因素法（OFAT）、Plackett-Burman设计（PBD）和Box Behnken设计（BBD），实现了WS在深层发酵状态下木聚糖酶的优化生产。培养温度（30℃）、WS和硫酸铵是OFAT推断出的促进木聚糖酶产生的关键决定因素。从PBD可知，WS浓度（%（/））、酵母提取物浓度（%（/））和生产培养基的初始pH值对所产生的木聚糖酶有显著影响（≤0.05）。通过岭分析路径最陡上升的估计岭定位，预测的WS浓度、生产培养基初始pH值和酵母提取物浓度水平分别为3.8%（/）、5.1和0.098%（/），可激发最终木聚糖酶水平（53.7 U/mL），提高8.95倍；实验室验证率为94.7%。目前的数据支持了使用这种生态友好、廉价且强大的方法将WS转化为增值产品木聚糖酶来扩大木聚糖酶生产规模。
