Macías D, Gañán Y
Department of Morphological Sciences, University of Extremadura, Badajoz, Spain.
Int J Dev Biol. 1991 Mar;35(1):63-7.
We have previously shown that removal of the apical ectodermal ridge of the third interdigital space of the chick leg bud at stages 28 and 29 is followed by the appearance of ectopic cartilage, which in the course of development gives rise to extra digits. These in vivo studies suggest that the pattern of skeletal morphogenesis in the limb depends on the inhibitory effect of the ectoderm. In the present study we tested whether zone polarizing activity (ZPA) exerted an effect on the pattern of experimental chondrogenesis in the interdigital space of the leg bud in stage 29 HH chick embryos. A small fragment of tissue from the ZPA in chick embryos in which ZPA activity was most intense was grafted onto the interdigital space in which chondrogenesis had previously been experimentally induced. No significant changes were observed in the course of differentiation of the recipient interdigital spaces with ZPA grafts, leading us to conclude that the graft failed to modify the morphogenetic fate of interdigital tissue.
我们之前已经表明，在第28和29阶段去除鸡胚腿芽第三趾间空间的顶端外胚层嵴后，会出现异位软骨，在发育过程中会产生额外的趾。这些体内研究表明，肢体中骨骼形态发生的模式取决于外胚层的抑制作用。在本研究中，我们测试了极化活性区（ZPA）是否对第29阶段HH鸡胚腿芽趾间空间的实验性软骨形成模式产生影响。将ZPA活性最强的鸡胚中ZPA的一小片组织移植到先前已通过实验诱导软骨形成的趾间空间。接受ZPA移植的趾间空间在分化过程中未观察到显著变化，这使我们得出结论，移植未能改变趾间组织的形态发生命运。
