Shimizu K, Ichikawa H, Miyoshi H, Ohki M, Kobayashi H, Maseki N, Kaneko Y
Department of Immunology and Virology, Saitama Cancer Center Research Institute, Japan.
Genes Chromosomes Cancer. 1991 May;3(3):163-7. doi: 10.1002/gcc.2870030302.
An 8;21 translocation is a common chromosome abnormality associated with acute myeloblastic leukemia with maturation (M2 of French-American-British (FAB) classification). We have isolated chromosome 21 Notl linking clones; pulsed field gel electrophoresis analysis with one clone (LL263) detected an altered fragment of Notl-digested leukemic cell DNA carrying t(8;21). The altered fragment was shown to be produced by the 8;21 translocation. The breakpoint in chromosome 21 was located about 13 kb to 100 kb proximal to the LL263 Notl site. Because the LL263 clone has a CpG island and is a short distance from the breakpoint, the clone itself may be considered as a candidate for part of the t(8;21) associated gene.
8;21易位是一种与急性髓细胞白血病伴成熟（法国-美国-英国（FAB）分类中的M2）相关的常见染色体异常。我们已经分离出21号染色体Notl连接克隆；用一个克隆（LL263）进行脉冲场凝胶电泳分析，检测到携带t(8;21)的Notl消化的白血病细胞DNA片段发生改变。该改变的片段显示是由8;21易位产生的。21号染色体的断裂点位于LL263 Notl位点近端约13 kb至100 kb处。由于LL263克隆有一个CpG岛且距离断裂点较近，该克隆本身可被视为t(8;21)相关基因一部分的候选者。
