Chen Lu, Rengifo Hernán R, Grigoras Cristian, Li Xiaoxu, Li Zengmin, Ju Jingyue, Koberstein Jeffrey T
Columbia University Department of Chemical Engineering 500 West 120th Street, New York, New York 10027, USA.
Biomacromolecules. 2008 Sep;9(9):2345-52. doi: 10.1021/bm800258g. Epub 2008 Aug 5.
We demonstrate a simple means to covalently bond DNA to both hard (i.e., glass and silicon wafers) and soft (i.e., polymeric) substrates that provides quantitative and precise control of the DNA areal density. The approach is based on spin coating an alkyne-end-functional diblock copolymer, alpha-alkyne-omega-Br-poly( tBA- b-MMA), that self-assembles on both types of substrates as an ordered monolayer and thereby directs alkyne groups to the surface. Azido-functionalized DNA is covalently linked to the alkyne functionalized substrates by means of a "click" reaction between azide and alkyne groups. The density of immobilized DNA can be quantitatively controlled by varying the parameters used for spin-coating the copolymer film, that is, solution concentration and rotational speed, or by varying the copolymer molecular weight. We find the yield of the DNA coupling reaction to be dependent on the nature of the polymer underlying the reactive alkyne functional groups, being higher for more hydrophilic polymers.
我们展示了一种将DNA共价连接到硬质（如玻璃和硅片）和软质（如聚合物）基底上的简单方法，该方法能对DNA的面密度进行定量且精确的控制。该方法基于旋涂一种炔端官能化的二嵌段共聚物，α-炔基-ω-溴-聚（叔丁基丙烯酸酯- b-甲基丙烯酸甲酯），它能在这两种类型的基底上自组装成有序的单分子层，从而将炔基导向表面。叠氮官能化的DNA通过叠氮基和炔基之间的“点击”反应与炔基官能化的基底共价连接。固定化DNA的密度可以通过改变用于旋涂共聚物薄膜的参数（即溶液浓度和转速）或改变共聚物分子量来定量控制。我们发现DNA偶联反应的产率取决于反应性炔基官能团下方聚合物的性质，对于亲水性更强的聚合物产率更高。
