Reznikoff William S
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, Massachusetts 02543, USA.
Annu Rev Genet. 2008;42:269-86. doi: 10.1146/annurev.genet.42.110807.091656.
Tn5 was one of the first transposons to be identified ( 10 ). As a result of Tn5's early discovery and its simple macromolecular requirements for transposition, the Tn5 system has been a very productive tool for studying the molecular mechanism of DNA transposition. These studies are of broad value because they offer insights into DNA transposition in general, because DNA transposition is a useful model with which to understand other types of protein-DNA interactions such as retroviral DNA integration and the DNA cleavage events involved in immunoglobulin gene formation, and because Tn5-derived tools are useful adjuncts in genetic experimentation.
Tn5是最早被鉴定出的转座子之一（10）。由于Tn5的早期发现及其转座所需的简单大分子条件，Tn5系统一直是研究DNA转座分子机制的非常有效的工具。这些研究具有广泛的价值，因为它们总体上为DNA转座提供了见解，因为DNA转座是一个有用的模型，可用于理解其他类型的蛋白质-DNA相互作用，如逆转录病毒DNA整合以及免疫球蛋白基因形成过程中涉及的DNA切割事件，还因为源自Tn5的工具在基因实验中是有用的辅助手段。
