Krysenko Sergii
Valent BioSciences, Biorational Research Center, 1910 Innovation Way, Suite 100, Libertyville, IL 60048, USA.
BioTech (Basel). 2025 Jan 2;14(1):3. doi: 10.3390/biotech14010003.
Organisms from the genus feature actinobacteria with complex developmental cycles and a great ability to produce a variety of natural products. These soil bacteria produce more than 2/3 of antibiotics used in medicine, and a large variety of bioactive compounds for industrial, medical and agricultural use. Although spp. have been studied for decades, the engineering of these bacteria remains challenging, and the available genetic tools are rather limited. Furthermore, most biosynthetic gene clusters in these bacteria are silent and require strategies to activate them and exploit their production potential. In order to explore, understand and manipulate the capabilities of spp. as a key bacterial for biotechnology, synthetic biology strategies emerged as a valuable component of research. Recent advancements in strategies for genetic manipulation of involving proposals of a large variety of synthetic components for the genetic toolbox, as well as new approaches for genome mining, assembly of genetic constructs and their delivery into the cell, allowed facilitation of the turnaround time of strain engineering and efficient production of new natural products at an industrial scale, but still have strain- and design-dependent limitations. A new perspective offered recently by technical advances in DNA sequencing, analysis and editing proposed strategies to overcome strain- and construct-specific difficulties in the engineering of . In this review, challenges and recent developments of approaches for engineering are discussed, an overview of novel synthetic biology strategies is provided and examples of successful application of new technologies in molecular genetic engineering of are highlighted.
来自该属的生物体具有放线菌，其发育周期复杂，并且具有产生多种天然产物的强大能力。这些土壤细菌产生了超过三分之二的医学用抗生素，以及大量用于工业、医学和农业的生物活性化合物。尽管对该属物种已经研究了数十年，但对这些细菌的工程改造仍然具有挑战性，可用的遗传工具相当有限。此外，这些细菌中的大多数生物合成基因簇是沉默的，需要激活它们并开发其生产潜力的策略。为了探索、理解和操纵该属物种作为生物技术关键细菌的能力，合成生物学策略成为该属研究的重要组成部分。最近在该属遗传操作策略方面的进展，包括为遗传工具箱提出了大量合成组件，以及基因组挖掘、遗传构建体组装及其导入细胞的新方法，使得菌株工程的周转时间得以缩短，并能够在工业规模上高效生产新的天然产物，但仍然存在菌株和设计依赖性的局限性。最近DNA测序、分析和编辑技术的进步提供了一个新的视角，提出了克服该属工程中菌株和构建体特异性困难的策略。在这篇综述中，讨论了该属工程方法面临的挑战和最新进展，概述了新型合成生物学策略，并突出了新技术在该属分子遗传工程中成功应用的实例。
