Lee Bong Hyo, Zhao Rong Jie, Moon Jin Young, Yoon Seong Shoon, Kim Jung-Ae, An Heeduk, Kwon Young Kyu, Hwang Meeyul, Choi Seong Hun, Shim Insop, Kim Bong Hyun, Yang Chae Ha
College of Oriental Medicine, Daegu Haany University, Daegu, South Korea.
Neurosci Lett. 2008 Oct 10;443(3):213-7. doi: 10.1016/j.neulet.2008.07.069. Epub 2008 Jul 31.
In our previous study we demonstrated that acupuncture at Shenmen (HT7) points suppressed a decrease of accumbal dopamine (DA) release in ethanol-withdrawn rats. Furthermore, here we found that it inhibited behavioral withdrawal signs of ethanol. In an effort to better understand the mechanisms underlying this inhibition, the potential role of GABA receptor system in acupuncture was investigated. Male Sprague-Dawley rats were treated with 3g/kg/day of ethanol (20%, w/v) or saline by intraperitoneal injection for 21 days. Following 48 or 72h of ethanol withdrawal, acupuncture was applied at bilateral HT7 for 1min. The selective GABA(A) antagonist bicuculline and the selective GABA(B) antagonist SCH 50911 were injected intraperitoneally 20min before acupuncture, respectively. Importantly, suppressive effects of acupuncture on DA deficiency were completely abolished by SCH 50911, but not by bicuculline, whereas ameliorating effects of acupuncture on ethanol withdrawal syndrome were completely blocked either by SCH 50911 or bicuculline. These results suggest that acupuncture at specific acupoint HT7 may normalize the DA release in the mesolimbic system and attenuate withdrawal syndrome through the GABA(B) receptor system in ethanol-withdrawn rats.
在我们之前的研究中，我们证明针刺神门穴（HT7）可抑制乙醇戒断大鼠伏隔核多巴胺（DA）释放的减少。此外，我们在此发现针刺可抑制乙醇的行为戒断症状。为了更好地理解这种抑制作用的潜在机制，我们研究了GABA受体系统在针刺中的作用。雄性Sprague-Dawley大鼠通过腹腔注射给予3g/kg/天的乙醇（20%，w/v）或生理盐水，持续21天。在乙醇戒断48或72小时后，针刺双侧HT7 1分钟。分别在针刺前20分钟腹腔注射选择性GABA(A)拮抗剂荷包牡丹碱和选择性GABA(B)拮抗剂SCH 50911。重要的是，针刺对DA缺乏的抑制作用被SCH 50911完全消除，但未被荷包牡丹碱消除，而针刺对乙醇戒断综合征的改善作用被SCH 50911或荷包牡丹碱完全阻断。这些结果表明，在乙醇戒断大鼠中，针刺特定穴位HT7可能通过GABA(B)受体系统使中脑边缘系统的DA释放正常化，并减轻戒断综合征。
