Kang J-H, Yang M-P
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Korea.
J Vet Intern Med. 2008 Sep-Oct;22(5):1166-73. doi: 10.1111/j.1939-1676.2008.0159.x. Epub 2008 Aug 4.
The use of soybean oil-based lipid emulsion (SO-based LE) in parenteral nutrition has been reported to impair neutrophil functions in humans and rodents. As yet, little is understood about the effects of SO-based LE on canine immune responses.
A short-term infusion with SO-based LE affects the phagocytic responses of canine peripheral blood polymorphonuclear neutrophilic leukocytes (PMNs).
Twenty-four healthy Beagle dogs.
Experimental study. Dogs were randomly assigned into groups of six and administered a 2-hour IV infusion with 0.9% NaCl solution or sufficient SO-based LE (INTRALIPOS 20%) to supply 40, 100, and 200% of the basal energy requirement (BER). PMN functions were determined after collecting blood samples before, immediately after, and 24 hours after the infusion.
None of the treatments significantly affected the phagocytic capacity of PMNs or circulating leukocyte numbers. The infusion providing 200% of BERs significantly reduced PMN oxidative burst activity, filamentous actin polymerization, and Cdc42 Rho guanosine triphosphatase activity immediately after its delivery. However, these functions were restored to pre-infusion values 24 hours after the infusion. The lower calorie infusions did not have these effects.
These results suggest that short-term infusions with a supraphysiological dose of SO-based LE may decrease the immune functions of canine PMNs. However, more long-term studies will be needed to extrapolate the effect of SO-based LE with clinically relevant doses in a practical situation.
据报道，在肠外营养中使用大豆油基脂质乳剂（SO基LE）会损害人类和啮齿动物的中性粒细胞功能。迄今为止，对于SO基LE对犬类免疫反应的影响了解甚少。
短期输注SO基LE会影响犬外周血多形核中性粒细胞（PMNs）的吞噬反应。
24只健康的比格犬。
实验研究。将犬随机分为每组6只，分别静脉输注2小时的0.9%氯化钠溶液或足量的SO基LE（英脱利匹特20%），以提供基础能量需求（BER）的40%、100%和200%。在输注前、输注后立即和输注后24小时采集血样后，测定PMN功能。
所有处理均未显著影响PMN的吞噬能力或循环白细胞数量。提供200%BER的输注在输注后立即显著降低了PMN的氧化爆发活性、丝状肌动蛋白聚合和Cdc42 Rho鸟苷三磷酸酶活性。然而，这些功能在输注后24小时恢复到输注前的值。较低热量的输注没有这些影响。
这些结果表明，短期输注超生理剂量的SO基LE可能会降低犬PMN的免疫功能。然而，需要更多的长期研究来推断在实际情况下临床相关剂量的SO基LE的效果。
