采用高效液相色谱法分离血清中的β-生育酚和γ-生育酚。
Separation of beta- and gamma-tocopherols in serum by high performance liquid chromatography.
作者信息
al-Meshari A A, Aleem M A
机构信息
Department of Obstetrics and Gynaecology, King Khalid University Hospital, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
出版信息
Biomed Chromatogr. 1991 Mar;5(2):83-5. doi: 10.1002/bmc.1130050208.
A simple and sensitive high performance liquid chromatographic method for the analysis of tocopherols (alpha, beta, gamma and delta) in serum with UV detection is described. In normal phase mode excellent separation of positional isomers (beta and gamma) was achieved by adjusting the proportion of isopropanol in the mobile phase. Detection limits for both beta and gamma were 0.62 mg/L and 0.42 and 1.2 mg/L for alpha and delta isomers, respectively. Measurement of all the tocopherol structural isomers (alpha, beta, gamma and delta) in a sample required only 400 microL of serum and each run was completed in less than 22 min.
描述了一种采用紫外检测的简单灵敏的高效液相色谱法，用于分析血清中的生育酚（α、β、γ和δ）。在正相模式下，通过调节流动相中异丙醇的比例，实现了位置异构体（β和γ）的出色分离。β和γ的检测限分别为0.62 mg/L，α和δ异构体的检测限分别为0.42和1.2 mg/L。测定样品中所有生育酚结构异构体（α、β、γ和δ）仅需400 μL血清，每次运行在不到22分钟内即可完成。
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