Pangallo Domenico, Drahovská Hana, Harichová Jana, Karelová Edita, Chovanová Katarína, Ferianc Peter, Turna Ján, Timko Jozef
Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovak Republic.
Antonie Van Leeuwenhoek. 2008 Nov;94(4):555-62. doi: 10.1007/s10482-008-9272-9. Epub 2008 Aug 6.
The properties of 166 environmental strains belonging to the seven enterococcal species were studied. Enterococci originated mainly from surface- and waste-waters. They were screened for the presence of enterocins, virulence factors, and antibiotic resistance. The presence of different enterocin genes (entA, entB, entP, ent31, entL50AB) was frequently observed in our enterococcal isolates, 109 strains contained at least one enterocin gene. The distribution of enterocin genes varied according to the species, the genes were present mainly in E. hirae and E. faecium. By enterocin spot assay, 10 isolates inhibited the growth of Listeria strains. To evaluate the pathogenic ability of isolates, the distribution of selected virulence genes (cylA, gelE and esp) was investigated, eleven strains were positive in some of these genes, five of them belonged to E. faecalis. Regarding the antibiotic resistance of isolates, only two strains were multiresistant and two strains (E. hirae and E. casseliflavus) were resistant to vancomycin.
对属于七种肠球菌的166株环境菌株的特性进行了研究。肠球菌主要源自地表水和废水。对它们进行了肠毒素、毒力因子和抗生素抗性的筛查。在我们分离的肠球菌中经常观察到不同肠毒素基因（entA、entB、entP、ent31、entL50AB）的存在，109株菌株至少含有一个肠毒素基因。肠毒素基因的分布因菌种而异，这些基因主要存在于海氏肠球菌和屎肠球菌中。通过肠毒素斑点试验，10株分离株抑制了李斯特菌菌株的生长。为了评估分离株的致病能力，研究了选定毒力基因（cylA、gelE和esp）的分布，其中11株在其中一些基因中呈阳性，其中5株属于粪肠球菌。关于分离株的抗生素抗性，只有两株具有多重抗性，两株（海氏肠球菌和格氏肠球菌）对万古霉素耐药。
