Poeta Patricia, Costa Daniela, Rodrigues Jorge, Torres Carmen
Universidade de Trás-os-Montes e Alto Douro, Departamento de Ciências Veterinárias, Vila Real, Portugal.
Avian Dis. 2006 Mar;50(1):64-8. doi: 10.1637/7394-061505R.1.
Seventy-six Enterococcus isolates (43 E. faecalis, 30 E. faecium, two E. durans, and one E. hirae) recovered from fecal samples of poultry in a slaughterhouse (one isolate per fecal sample and one fecal sample per lot of animals) were studied for bacteriocin production and for the presence of genes encoding bacteriocins and virulence factors. The presence of genes encoding virulence factors (cpd, geE, fsr, ace, agg, and esp) and bacteriocins (entA, entB, entP, entQ, entAS-48, entL50A/B, cyl, and bac31) were studied by polymerase chain reaction in all enterococci. At least two virulence genes were detected in all 43 E. faecalis isolates, cpd and gelE being the most frequently detected genes (97.7%) followed by ace (62.8%), agg (39.5%), fsr (27.9%), and esp (2.3%). No virulence genes were detected in the other enterococcal species with the exception of one E. faecium and one E. durans isolates that harbored the gelE gene. Antimicrobial activity against eight indicator bacteria (including Listeria monocytogenes) was assayed in the enterococci, and 23 (30.3%) showed inhibitory activity against L. monocytogenes, the other 22 enterococci showing activity against indicator bacteria other than L. monocytogenes. Only the entA, entB, and cyl genes were detected in our study (entA + entB in nine E. faecium isolates and the cyl gene in seven E. faecalis isolates). A wide variety of virulence genes have been detected in fecal E. faecalis isolates from poultry, but not in the other enterococcal species. However, the presence of known bacteriocin structural genes is associated more with the E. faecium species.
从一家屠宰场的家禽粪便样本中分离出76株肠球菌（43株粪肠球菌、30株屎肠球菌、2株耐久肠球菌和1株希拉肠球菌）（每个粪便样本分离1株，每批动物取1个粪便样本），对其进行细菌素产生情况以及编码细菌素和毒力因子的基因检测。通过聚合酶链反应研究所有肠球菌中编码毒力因子（cpd、geE、fsr、ace、agg和esp）和细菌素（entA、entB、entP、entQ、entAS - 48、entL50A/B、cyl和bac31）的基因的存在情况。在所有43株粪肠球菌分离株中至少检测到两个毒力基因，cpd和gelE是最常检测到的基因（97.7%），其次是ace（62.8%）、agg（39.5%）、fsr（27.9%）和esp（2.3%）。除了1株携带gelE基因的屎肠球菌和1株耐久肠球菌分离株外，在其他肠球菌种类中未检测到毒力基因。对肠球菌进行了针对8种指示菌（包括单核细胞增生李斯特菌）的抗菌活性检测，23株（30.3%）对单核细胞增生李斯特菌显示出抑制活性，另外22株肠球菌对除单核细胞增生李斯特菌外的指示菌有活性。在我们的研究中仅检测到entA、entB和cyl基因（9株屎肠球菌分离株中entA + entB，7株粪肠球菌分离株中cyl基因）。在家禽粪便粪肠球菌分离株中检测到多种毒力基因，但在其他肠球菌种类中未检测到。然而，已知细菌素结构基因的存在更多地与屎肠球菌种类相关。
