Ikegami S, Nihonmatsu I, Kawamura H
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Brain Res. 1991 May 10;548(1-2):187-95. doi: 10.1016/0006-8993(91)91121-g.
Two types of cholinergic neurons were transplanted into the hippoccampus of adult rats chemically damaged by lateral ventricular administration of AF64A, a cholinergic neurotoxin, and the effects were compared with respect to their ability to reinnervate the hippocampus and to repair behavioral deficit. Pieces of brain tissue containing the nucleus basalis magnocellularis (NBM) or the striatum were taken for grafting from 17-day rat fetuses. About 3 months after transplantation, the rats with bilateral NBM grafts showed significant amelioration in radial-arm maze performance and habituation to a novel environment in an open field box, although they had not recovered to the control level. In rats with NBM grafts that showed a good performance, there were surviving grafts and many ingrowths of AChE-positive fibers in the hippocampus. By contrast, rats with striatal grafts showed hardly any significant improvement in these behavioral measures. The AChE staining revealed poor outgrowth of the striatal grafts into the hippocampus. These results indicate that grafting of NBM cholinergic neurons, which are anatomically similar to septal neurons, into the hippocampus produces a partial restorative effect on the cognitive impairment associated with hypofunction of the septohippocampal system.
将两种胆碱能神经元移植到经侧脑室注射胆碱能神经毒素AF64A化学损伤的成年大鼠海马中，并比较它们重新支配海马和修复行为缺陷能力的效果。从17日龄大鼠胎儿中获取含有大细胞基底核（NBM）或纹状体的脑组织块用于移植。移植后约3个月，双侧NBM移植的大鼠在放射状臂迷宫任务表现以及在旷场箱中对新环境的适应能力方面有显著改善，尽管它们尚未恢复到对照水平。在表现良好的NBM移植大鼠中，有存活的移植组织，并且海马中有许多乙酰胆碱酯酶（AChE）阳性纤维向内生长。相比之下，纹状体移植的大鼠在这些行为指标上几乎没有任何显著改善。AChE染色显示纹状体移植组织向海马的生长较差。这些结果表明，将在解剖学上与隔区神经元相似的NBM胆碱能神经元移植到海马中，对与隔海马系统功能减退相关的认知障碍产生部分恢复作用。
