Wang Wen-Xia, Sun Shan-Zhen, Guo Xiao-Ling, Song Ying
Department.of Oral Pathology, School of Stomatology, Shandong University, Jinan 250012, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Feb;43(2):101-4.
To study the effect of curcumin on invasion and migration of the tongue squamous cell line Tca8113.
Tca8113 cells were treated with curcumin (0 - 100 micromol/L) for 24 h and the conditional medium was collected. The gelatinases - matrix metalloproteinase -2 and -9 (MMP-2, -9) in the conditional medium were detected by gelatin zymography. The cell invasion and migration model in vitro was conducted using transwell chamber with or without matrigel. Using this model, the effects of 50 micromol/L curcumin on invasion and migration of Tca8113 were detected.
Curcumin reduced the activities of MMP-2 and MMP-9 on a dose-dependent manner. Curcumin can suppress cell invasion and migration significantly (P <0.01).
Curcumin can suppress Tca8113 invasion and migration by reducing the activities of MMP-2 and MMP-9.
研究姜黄素对舌鳞状细胞系Tca8113侵袭和迁移的影响。
用姜黄素（0 - 100微摩尔/升）处理Tca8113细胞24小时，收集条件培养基。用明胶酶谱法检测条件培养基中的明胶酶 - 基质金属蛋白酶 -2和 -9（MMP - 2， -9）。使用有或没有基质胶的transwell小室建立体外细胞侵袭和迁移模型。利用该模型，检测50微摩尔/升姜黄素对Tca8113侵袭和迁移的影响。
姜黄素以剂量依赖性方式降低MMP - 2和MMP - 9的活性。姜黄素能显著抑制细胞侵袭和迁移（P <0.01）。
姜黄素可通过降低MMP - 2和MMP - 9的活性来抑制Tca8113的侵袭和迁移。
