Zhang Xi-Ying, Zhang Yan-Jiao, Chen Xiu-Lan, Qin Qi-Long, Zhao Dian-Li, Li Tie-Gang, Dang Hong-Yue, Zhang Yu-Zhong
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, China.
FEMS Microbiol Lett. 2008 Oct;287(1):108-12. doi: 10.1111/j.1574-6968.2008.01299.x. Epub 2008 Aug 5.
A Gram-negative, nonmotile, aerobic and oxidase- and catalase-positive bacterium, designated D25T, was isolated from the deep-sea sediments of the southern Okinawa Trough area. Phylogenetic analyses of 16S rRNA gene sequences showed that strain D25T fell within the genus Myroides, with 99.2%, 96.0% and 93.4% sequence similarities to the only three recognized species of Myroides. However, the DNA-DNA similarity value between strain D25T and its nearest neighbour Myroides odoratimimus JCM 7460T was only 49.9% (<70%). Several phenotypic properties could be used to distinguish strain D25T from other Myroides species. The main cellular fatty acids of strain D25T were iso-C15:0, iso-C17:1omega9c, iso-C17:03-OH and Summed Feature 3 (comprising C16:1omega7c and/or iso-C15:02-OH). The major respiratory quinone was MK-6. The DNA G+C content was 33.0 mol%. The results of the polyphasic taxonomy analysis suggested that strain D25T represents a novel species of the genus Myroides, for which the name Myroides profundi sp. nov. is proposed. The type strain is D25T (=CCTCC M 208030T=DSM 19823T).
从冲绳海槽南部地区的深海沉积物中分离出一株革兰氏阴性、无动力、需氧、氧化酶和过氧化氢酶阳性的细菌，命名为D25T。16S rRNA基因序列的系统发育分析表明，菌株D25T属于Myroides属，与Myroides属仅有的三个已确认物种的序列相似性分别为99.2%、96.0%和93.4%。然而，菌株D25T与其最接近的亲缘种Myroides odoratimimus JCM 7460T之间的DNA-DNA相似性值仅为49.9%（<70%）。一些表型特征可用于区分菌株D25T与其他Myroides属物种。菌株D25T的主要细胞脂肪酸为异C15:0、异C17:1ω9c、异C17:0 3-OH和特征性总和3（包含C16:1ω7c和/或异C15:0 2-OH）。主要呼吸醌为MK-6。DNA G+C含量为33.0 mol%。多相分类分析结果表明，菌株D25T代表Myroides属的一个新物种，为此提出新名称Myroides profundi sp. nov.。模式菌株为D25T（=CCTCC M 208030T=DSM 19823T）。
