Edelstein P S, Thompson S M, Davies R J
University of California, San Diego 92103.
Cancer Res. 1991 Aug 15;51(16):4492-4.
Intracellular calcium [(Ca2+)i] was measured in human colonic epithelia derived from control mucosa from noncancer patients, from grossly "normal" mucosa taken 10-30 cm proximal to primary colorectal cancers, and from colorectal cancers. (Ca2+)i was measured first in cells bathed by calcium-free solution and again after adding 1 mM calcium. Compared to control mucosa, (Ca2+)i was 27% lower in cancer cells in the presence of 1 mM extracellular calcium, whereas (Ca2+)i was elevated more than 3-fold in the adjacent "normal" mucosa from cancer patients in both media. These results suggest altered (Ca2+)i levels in malignant cells and the presence of a "field defect" in (Ca2+)i regulation in "normal" colonic mucosa adjacent to colorectal cancers.
在来自非癌症患者对照黏膜、距原发性结直肠癌近端10 - 30厘米处获取的大体“正常”黏膜以及结直肠癌的人结肠上皮细胞中测量细胞内钙（[Ca2+]i）。首先在无钙溶液中孵育的细胞中测量[Ca2+]i，然后在添加1 mM钙后再次测量。与对照黏膜相比，在存在1 mM细胞外钙的情况下，癌细胞中的[Ca2+]i降低了27%，而在两种培养基中，癌症患者相邻“正常”黏膜中的[Ca2+]i升高了3倍以上。这些结果表明恶性细胞中[Ca2+]i水平改变，且在结直肠癌相邻的“正常”结肠黏膜中存在[Ca2+]i调节的“场缺陷”。
