Kimura Masanobu, Imaoka Koichi, Suzuki Michio, Kamiyama Tsuneo, Yamada Akio
Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.
J Vet Med Sci. 2008 Jul;70(7):707-9. doi: 10.1292/jvms.70.707.
A microplate agglutination test (MAT) was compared with the tube agglutinin test (TAT), a standard test for the diagnosis of Brucella canis, in terms of the sensitivity and specificity. The results showed that MAT was more sensitive, simpler to perform and easier to read the results than TAT. On top of that the MAT allows us to handle a larger number of samples at once. Using this method we conducted sero-surveillance of the prevalence of B. canis in dogs kept in an Animal Shelter located in Kanagawa Prefecture. Twelve of 485 (2.5%) showed seropositive against B. canis. These results indicate that B. canis infection in dogs is still occurring in Japan.
就敏感性和特异性而言，对用于诊断犬布鲁氏菌病的标准试验——试管凝集试验（TAT）与微量板凝集试验（MAT）进行了比较。结果表明，与TAT相比，MAT更敏感，操作更简单，结果读取更容易。除此之外，MAT使我们能够一次处理更多数量的样本。使用这种方法，我们对神奈川县一家动物收容所饲养的犬只中犬布鲁氏菌的流行情况进行了血清学监测。485只犬中有12只（2.5%）对犬布鲁氏菌呈血清阳性。这些结果表明，犬布鲁氏菌感染在日本仍有发生。
