Makiyama N, Matsui H, Tsuji H, Ichimura K
Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, Faculty of Medicine, Japan.
Clin Exp Metastasis. 1991 Jul-Aug;9(4):411-25. doi: 10.1007/BF01769360.
High- and low-metastatic clones established from poorly differentiated murine sarcoma (RCT sarcoma) spontaneously developed in C3H/He mice were used in this study. Attachment and invasion, which are factors likely related to metastatic ability, of these clones were investigated in a three-dimensional culture system using embryonic chick heart fragments and tumor cell aggregates. The effects of Nocodazole, which interferes with the function of microtubules, and cytochalasin B, which affects the actin assembly in cytoplasmic extensions, on these factors were also examined. Metastatic ability was correlated with attachment and invasiveness of RCT sarcoma. Invasiveness of tumor cells was suppressed by the addition of Nocodazole to the culture medium. Since the disappearance of cell polarity, resulting from the inhibition of tubulin assembly, was found to coincide with the suppression of invasion, directional migration is considered to be involved in the invasion of tumor cells. Cytochalasin B caused a marked decrease in the ratio of attachment of tumor aggregates to embryonic chick heart fragments. The increased ability of invasion and attachment of RCT(+) cells compared with RCT(-) cells seems to contribute to their propensity to metastasize.
本研究使用了从C3H/He小鼠自发形成的低分化鼠肉瘤（RCT肉瘤）中建立的高转移和低转移克隆。在使用胚胎鸡心脏碎片和肿瘤细胞聚集体的三维培养系统中，研究了这些克隆的附着和侵袭情况，这两种因素可能与转移能力有关。还研究了干扰微管功能的诺考达唑和影响细胞质延伸中肌动蛋白组装的细胞松弛素B对这些因素的影响。转移能力与RCT肉瘤的附着和侵袭性相关。向培养基中添加诺考达唑可抑制肿瘤细胞的侵袭性。由于发现微管蛋白组装抑制导致的细胞极性消失与侵袭抑制同时发生，因此认为定向迁移参与了肿瘤细胞的侵袭。细胞松弛素B使肿瘤聚集体与胚胎鸡心脏碎片的附着率显著降低。与RCT(-)细胞相比，RCT(+)细胞侵袭和附着能力的增强似乎有助于它们的转移倾向。
