Matsui H, Tatezaki S, Tsuji H, Ochiai H
Department of Orthopaedics, Toyama Medical and Pharmaceutical University, Japan.
J Cancer Res Clin Oncol. 1989;115(1):9-16. doi: 10.1007/BF00391593.
We have established low- and high-metastatic clones, named RCT(-) and RCT(+) cells, respectively, from the RCT (Radiological, Chiba, and Toyama) sarcoma spontaneously developed in a C3H/He male mouse by the limiting-dilution method in vitro or by the combination of the lung passages and limiting-dilution methods. After 20 serial passages in vitro, the metastatic potential of each clone did not alter. Morphologically, both cells were spindle-shaped, but RCT(+) cells were slightly thicker and larger than RCT(-) cells. The organization of actin-containing filaments was slightly poorer in RCT(+) cells than that in RCT(-) cells. Marked differences were observed in their growth characteristics and adhesiveness to plastic or collagen-coated surfaces, that is, RCT(+) cells grew more slowly but could adhere more rapidly and firmly to the surfaces than RCT(-) cells. RCT(+) cells were agglutinated by all lectins used but several lectins could not agglutinate RCT(-) cells. These results could be a reflection of the difference in oligosaccharide residues on the surface of each cell and, in part, might reflect the difference in organization of the actin-containing filaments that regulate the mobility of lectin receptors. No significant difference between these cell clones was noted in their sensitivity to natural-killer-cell-mediated cytotoxicity in vitro. RCT(-) and RCT(+) cells are considered to be the most useful experimental model for the study of the certain sarcomas.
我们通过体外有限稀释法或肺转移结合有限稀释法，从一只C3H/He雄性小鼠自发形成的RCT（放射学、千叶和富山）肉瘤中，分别建立了低转移和高转移克隆，命名为RCT(-)和RCT(+)细胞。在体外连续传代20次后，每个克隆的转移潜能没有改变。形态学上，两种细胞均呈纺锤形，但RCT(+)细胞比RCT(-)细胞略粗且大。RCT(+)细胞中含肌动蛋白丝的组织化程度比RCT(-)细胞略差。在它们的生长特性以及对塑料或胶原包被表面的黏附性方面观察到显著差异，即RCT(+)细胞生长较慢，但比RCT(-)细胞能更快更牢固地黏附于表面。所有使用的凝集素均可凝集RCT(+)细胞，但几种凝集素不能凝集RCT(-)细胞。这些结果可能反映了每个细胞表面寡糖残基的差异，并且在一定程度上可能反映了调节凝集素受体流动性的含肌动蛋白丝组织化的差异。在体外对自然杀伤细胞介导的细胞毒性的敏感性方面，这些细胞克隆之间未观察到显著差异。RCT(-)和RCT(+)细胞被认为是研究某些肉瘤最有用的实验模型。
